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          • Primary Antibodies ›
          • Cullin 1 Antibodies

          Invitrogen

          Cullin 1 Polyclonal Antibody

          Advanced Verification
          57 Published Figures
          60 References
          View all (23) Cullin 1 antibodies

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          Cite Cullin 1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (57)
          • Advanced Verification (1)
          Cullin 1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Cullin 1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 60

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          Cullin 1 Antibody (71-8700) in WB

          Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), NTERA-2 (Lane 2), MCF-7 (Lane 3), A549 (Lane 4), and Caco-2 (Lane 5). The blots were probed with Anti- CUL1 Rabbit Polyclonal Antibody (Product # 71-8700, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 89 kDa band corresponding to CUL1 was observed across cell lines tested. Additionally ~68 kDa band was obse... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Western Blot (WB)
          Cullin 1 Antibody in Immunoprecipitation (IP)
          Cullin 1 Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Cullin 1 Polyclonal Antibody

          Product Details

          71-8700

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 45 publications 45 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          View 9 publications 9 publications

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Fruit fly, Human, Insect, Mouse, Non-human primate, Virus

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          An 11 amino acid synthetic peptide derived from the extreme C-terminus of the human CUL1 protein. This peptide sequence is identical to Drosophila CUL1 (LIN-19) in 9/11 positions and in 7/11 residues with the CUL1 C. elegans and Dictyostelium discoidium homologs
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2534002

          Target Information

          CUL1 gene ontology annotations related to this gene include animal organ morphogenesis; apoptotic process; cell proliferation; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AT4G02570; ATCUL1; AXR6; CUL-1; ETA1; ICU13; unnamed protein product

          View more View less

          UniProt ID: (Human) Q13616

          View more View less

          Entrez Gene ID: (Human) 8454

          View more View less

          Function(s)
          protein binding ubiquitin protein ligase binding ubiquitin ligase complex scaffold activity
          Process(es)
          G1/S transition of mitotic cell cycle regulation of transcription, DNA-templated ubiquitin-dependent protein catabolic process cellular iron ion homeostasis regulation of mitotic cell cycle regulation of cell cycle process regulation of centrosome duplication neural crest cell differentiation protein ubiquitination regulation of BMP signaling pathway SCF-dependent proteasomal ubiquitin-dependent protein catabolic process regulation of TOR signaling cellular response to oxidative stress regulation of circadian rhythm regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of inflammatory response centrosome duplication regulation of cell cycle limb development cilium morphogenesis protein K48-linked ubiquitination intrinsic apoptotic signaling pathway regulation of mitophagy regulation of xenophagy regulation of DNA damage checkpoint
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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