Invitrogen

Cullin 2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

34 Published Figures

26 References

View all (12) Cullin 2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 44

Cullin 2 Antibody (51-1800) in WB

Western blot analysis of CUL-2 was performed by loading 20 µg of HEL 92.1.7 (lane1), Rat Heart (lane2), K562 (lane3), MCF7 (lane4), HeLa (lane5) and Caco-2 (lane6) lysate using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. CUL-2 was detected at ~ 83 kDa using C... View More

Product Image Gallery

Cullin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CUL-2 showing staining in the cytoplasm and nucleus of paraffin-embedded human Ovarian carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CUL-2 Rabbit Polyclonal antibody (Product # 51-1800) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Cullin 2 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of CUL2 was performed with 5 µg of the CUL2 Rabbit Polyclonal Antibody (Product # 51-1800) on cell extract from HEL 92.1.7 using the Dynabeads® Protein A Immunoprecipitation Kit (10006D). Normal Rabbit IgG was used as a negative IP control. Subsequently, western blot analysis was performed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred using iBlot® 2 Dry Blotting System (IB21001) to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature on a rocking platform. CUL2 was detected at ~ 80 kDa using CUL2 Rabbit Polyclonal Antibody (Product # 51-1800) at 2-3 µg/mL in 5 % skim milk at 37°C for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Lane 1 represents 10 % of the total cell extract (input), Lane 2 is the IP performed with Rabbit IgG and Lane 3 represents IP performed with CUL2 Rabbit Polyclonal Antibody (Product # 51-1800).

Cullin 2 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig 6 ZTA transcription activation domain mediates interactions with CAND1 and Cullins. (A) Schematic of ZTA transcription activation (TA) indicating position of sumoylation site at K12 and Cul5 box 52-LPEP-54. (B) Luciferase assay for BHLF1-Luc cotransfected with expression vector for ZTA (WT), K12A, or EP53/54AA (MUT) in 293T cells treated with DMSO (black bars) or C60 (grey bars) for 48 hrs. (C) Western blot of 293T cells transfected with ZTA (WT), K12A, or EP53/54AA treated with DMSO or C60 for 48 hrs, as described in panel B. (D-E) 293T cells transfected with FL-CTRL, FL-WT-ZTA, or FL-MUT-ZTA were treated with DMSO or C60 for 48 hrs, and then subject to IP with FLAG and probed with antibodies for Cul 1, Cul 2, CAND1, FLAG, or Actin (D) . 10% of input was analyzed by Western for Cul 1, Cul 2, CAND1, FLAG, and Actin (E) . (F-G) 293T cells were transfected as in D-E, and subject to IP with either Cul 1, Cul 2, or FLAG, and then analyzed by Western blot with antibody to FLAG (F) . 10% of input was analyzed by Western for Cul 1, Cul 2, FLAG, and Actin (G) .

Cullin 2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NTERA-2 cells were transfected with Cullin 2 siRNA and loss of signal was observed in Western Blot using Anti- Cullin 2 Polyclonal Antibody (Product # 51-1800). {KD}

Product Details

51-1800

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 21 publications 21 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

2-3 µg/mL

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Insect, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminus of the human CUL2

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533898

Target Information

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cullin neddylation inhibitor attenuates hyperglycemia by enhancing hepatic insulin signaling through insulin receptor substrate stabilization.

Proceedings of the National Academy of Sciences of the United States of America

Chen C,Gu L,Matye DJ,Clayton YD,Hasan MN,Wang Y,Friedman JE,Li T

51-1800 was used in Knock Down to suggest that pharmacological inhibition of cullin neddylation represents a therapeutic approach for improving hepatic insulin signaling and lowering blood glucose.

Tue Feb 08 00:00:00 EST 2022

Target protein localization and its impact on PROTAC-mediated degradation.

Cell chemical biology

Simpson LM,Glennie L,Brewer A,Zhao JF,Crooks J,Shpiro N,Sapkota GP

51-1800 was used in Western Blotting to suggest that the subcellular context of the POI can influence the efficacy of PROTAC-mediated POI degradation.

Thu Oct 20 00:00:00 EDT 2022

Desmosomal COP9 regulates proteome degradation in arrhythmogenic right ventricular dysplasia/cardiomyopathy.

The Journal of clinical investigation

Liang Y,Lyon RC,Pellman J,Bradford WH,Lange S,Bogomolovas J,Dalton ND,Gu Y,Bobar M,Lee MH,Iwakuma T,Nigam V,Asimaki A,Scheinman M,Peterson KL,Sheikh F

51-1800 was used in Western Blot to identify a desmosomal resident regulatory complex that restricted desmosomal proteome degradation and disease.

Tue Jun 01 00:00:00 EDT 2021

Quantitative analyses for effects of neddylation on CRL2^VHL substrate ubiquitination and degradation.

Protein science : a publication of the Protein Society

Wang K,Reichermeier KM,Liu X

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Mon Nov 01 00:00:00 EDT 2021

Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System.

Cell chemical biology

Simpson LM,Macartney TJ,Nardin A,Fulcher LJ,Röth S,Testa A,Maniaci C,Ciulli A,Ganley IG,Sapkota GP

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Thu Sep 17 00:00:00 EDT 2020

Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System.

Cell chemical biology

Simpson LM,Macartney TJ,Nardin A,Fulcher LJ,Röth S,Testa A,Maniaci C,Ciulli A,Ganley IG,Sapkota GP

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Thu Sep 17 00:00:00 EDT 2020

Small molecule perturbation of the CAND1-Cullin1-ubiquitin cycle stabilizes p53 and triggers Epstein-Barr virus reactivation.

PLoS pathogens

Tikhmyanova N,Tutton S,Martin KA,Lu F,Kossenkov AV,Paparoidamis N,Kenney S,Salvino JM,Lieberman PM

51-1800 was used in Western Blotting to understand the mechanism of action of C60, with the aim of identifying new approaches for treatment of Epstein-Barr Virus associated tumours.

Sat Jul 01 00:00:00 EDT 2017

Small molecule perturbation of the CAND1-Cullin1-ubiquitin cycle stabilizes p53 and triggers Epstein-Barr virus reactivation.

PLoS pathogens

Tikhmyanova N,Tutton S,Martin KA,Lu F,Kossenkov AV,Paparoidamis N,Kenney S,Salvino JM,Lieberman PM

51-1800 was used in Western Blotting to understand the mechanism of action of C60, with the aim of identifying new approaches for treatment of Epstein-Barr Virus associated tumours.

Sat Jul 01 00:00:00 EDT 2017

Small molecule perturbation of the CAND1-Cullin1-ubiquitin cycle stabilizes p53 and triggers Epstein-Barr virus reactivation.

PLoS pathogens

Tikhmyanova N,Tutton S,Martin KA,Lu F,Kossenkov AV,Paparoidamis N,Kenney S,Salvino JM,Lieberman PM

51-1800 was used in Western Blotting to understand the mechanism of action of C60, with the aim of identifying new approaches for treatment of Epstein-Barr Virus associated tumours.

Sat Jul 01 00:00:00 EDT 2017

An affinity-directed protein missile system for targeted proteolysis.

Open biology

Fulcher LJ,Macartney T,Bozatzi P,Hornberger A,Rojas-Fernandez A,Sapkota GP

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Sat Oct 01 00:00:00 EDT 2016

Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle.

eLife

Mosadeghi R,Reichermeier KM,Winkler M,Schreiber A,Reitsma JM,Zhang Y,Stengel F,Cao J,Kim M,Sweredoski MJ,Hess S,Leitner A,Aebersold R,Peter M,Deshaies RJ,Enchev RI

51-1800 was used in western blot to study the cullin-RING ubiquitin ligase deneddylation cycle and COP9-signalosome activation by structural and kinetic analysis

Thu Mar 31 00:00:00 EDT 2016

Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling.

The Journal of biological chemistry

Heir P,Srikumar T,Bikopoulos G,Bunda S,Poon BP,Lee JE,Raught B,Ohh M

51-1800 was used in Immunoprecipitation to characterise the locations and effects of erythropoietin receptor hydroxylation by prolyl hydroxylase 3.

Fri Apr 01 00:00:00 EDT 2016

Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations.

The Journal of biological chemistry

Sandoval D,Hill S,Ziemba A,Lewis S,Kuhlman B,Kleiger G

51-1800 was used in western blot to study the interaction between ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase.

Fri Jan 09 00:00:00 EST 2015

Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations.

The Journal of biological chemistry

Sandoval D,Hill S,Ziemba A,Lewis S,Kuhlman B,Kleiger G

51-1800 was used in western blot to study the interaction between ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase.

Fri Jan 09 00:00:00 EST 2015

CCDC22 deficiency in humans blunts activation of proinflammatory NF-κB signaling.

The Journal of clinical investigation

Starokadomskyy P,Gluck N,Li H,Chen B,Wallis M,Maine GN,Mao X,Zaidi IW,Hein MY,McDonald FJ,Lenzner S,Zecha A,Ropers HH,Kuss AW,McGaughran J,Gecz J,Burstein E

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Wed May 01 00:00:00 EDT 2013

DCNL1 functions as a substrate sensor and activator of cullin 2-RING ligase.

Molecular and cellular biology

Heir P,Sufan RI,Greer SN,Poon BP,Lee JE,Ohh M

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Mon Apr 01 00:00:00 EDT 2013

The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.

The Biochemical journal

Bett JS,Ibrahim AF,Garg AK,Kelly V,Pedrioli P,Rocha S,Hay RT

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Mon Apr 15 00:00:00 EDT 2013

NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway.

Nature structural & molecular biology

den Besten W,Verma R,Kleiger G,Oania RS,Deshaies RJ

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Sun Apr 01 00:00:00 EDT 2012

UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1α accumulation.

BMC biology

Bandau S,Knebel A,Gage ZO,Wood NT,Alexandru G

Thu Apr 26 00:00:00 EDT 2012

Neddylation-induced conformational control regulates cullin RING ligase activity in vivo.

Journal of molecular biology

Boh BK,Smith PG,Hagen T

51-1800 was used in western blot to study the effect of neddylation on Cul2 and Cul3 structure.

Fri Jun 03 00:00:00 EDT 2011

Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity.

Molecular biology of the cell

Choo YY,Boh BK,Lou JJ,Eng J,Leck YC,Anders B,Smith PG,Hagen T

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Thu Dec 01 00:00:00 EST 2011

COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.

The Journal of biological chemistry

Mao X,Gluck N,Chen B,Starokadomskyy P,Li H,Maine GN,Burstein E

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Fri Sep 16 00:00:00 EDT 2011

COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.

The Journal of biological chemistry

Mao X,Gluck N,Chen B,Starokadomskyy P,Li H,Maine GN,Burstein E

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Fri Sep 16 00:00:00 EDT 2011

COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.

The Journal of biological chemistry

Mao X,Gluck N,Chen B,Starokadomskyy P,Li H,Maine GN,Burstein E

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Fri Sep 16 00:00:00 EDT 2011

GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA.

Genes & development

Mao X,Gluck N,Li D,Maine GN,Li H,Zaidi IW,Repaka A,Mayo MW,Burstein E

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Wed Apr 01 00:00:00 EDT 2009

Substrate-mediated regulation of cullin neddylation.

The Journal of biological chemistry

Chew EH,Hagen T

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Fri Jun 08 00:00:00 EDT 2007

Substrate-mediated regulation of cullin neddylation.

The Journal of biological chemistry

Chew EH,Hagen T

Published figure using Cullin 2 polyclonal antibody (Product # 51-1800) in Western Blot

Fri Jun 08 00:00:00 EDT 2007

Renal cell carcinoma risk in type 2 von Hippel-Lindau disease correlates with defects in pVHL stability and HIF-1alpha interactions.

Oncogene

Knauth K,Bex C,Jemth P,Buchberger A

Thu Jan 19 00:00:00 EST 2006

Renal cell carcinoma risk in type 2 von Hippel-Lindau disease correlates with defects in pVHL stability and HIF-1alpha interactions.

Oncogene

Knauth K,Bex C,Jemth P,Buchberger A

Thu Jan 19 00:00:00 EST 2006

Tumor suppression by the von Hippel-Lindau protein requires phosphorylation of the acidic domain.

The Journal of biological chemistry

Lolkema MP,Gervais ML,Snijckers CM,Hill RP,Giles RH,Voest EE,Ohh M

Fri Jun 10 00:00:00 EDT 2005

SOCS-1 localizes to the microtubule organizing complex-associated 20S proteasome.

Molecular and cellular biology

Vuong BQ,Arenzana TL,Showalter BM,Losman J,Chen XP,Mostecki J,Banks AS,Limnander A,Fernandez N,Rothman PB

51-1800 was used in Western Blotting to show that SOCS-1 localises to the microtubule organising complex (MTOC) and the MTOC-associated 20S proteasome.

Fri Oct 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CUL-2; Cullin-2; MGC131970; OTTHUMP00000019437; testis secretory sperm-binding protein Li 238E

View more View less

Gene Aliases: 1300003D18Rik; 4932411N15Rik; AI327301; CUL2; mKIAA4106

View more View less

UniProt ID: (Human) Q13617, (Mouse) Q9D4H8

View more View less

Entrez Gene ID: (Human) 8453, (Rat) 361258, (Mouse) 71745

View more View less

Molecular Function: ubiquitin-protein ligase

Function(s)

G1/S transition of mitotic cell cycle cell cycle arrest negative regulation of cell proliferation viral process protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of transcription from RNA polymerase II promoter in response to hypoxia intrinsic apoptotic signaling pathway ubiquitin-dependent protein catabolic process biological_process protein ubiquitination protein catabolic process

Process(es)

protein binding ubiquitin protein ligase binding protein complex binding ubiquitin protein ligase activity ubiquitin-protein transferase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Cullin 2 Polyclonal Antibody

Cullin 2 Antibody (51-1800) in WB

Product Details

51-1800

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!