Invitrogen

Cullin 4B Polyclonal Antibody

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Datasheet

Protocols

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Cullin 4B Antibody in Immunocytochemistry (ICC/IF) — Cullin 4B Polyclonal Antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cullin 4b protein stained by Cullin 4B Polyclonal Antibody (Product # PA5-78252) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Cullin 4B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Cullin 4B Polyclonal Antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. Cullin 4B Polyclonal Antibody (Product # PA5-78252) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Cullin 4B Antibody in Western Blot (WB) — Cullin 4B Polyclonal Antibody detects Cullin 4b protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg C8D30 whole cell lysate/extract. C. 30 µg NIH-3T3 whole cell lysate/extract. D. 30 µg Raw 264.7 whole cell lysate/extract. E. 30 µg C2Cl2 whole cell lysate/extract.5 % SDS-PAGE. Cullin 4B Polyclonal Antibody (Product # PA5-78252) dilution: 1:1,000.

Product Details

PA5-78252

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human Cullin 4b. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735693

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, A375, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2

Predicted reactivity: Mouse (93%), Rat (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.