Bioss

Cux2 Polyclonal Antibody

View all (4) CUX2 antibodies

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Questions & Answers

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Cux2 Antibody in Immunocytochemistry (ICC/IF) — Tissue/cell: U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with Cux2 Polyclonal Antibody, Unconjugated (bs-11832R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5 µg/mL, blue) was used to stain the cell nuclei.

Cux2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cux2 Polyclonal Antibody, Unconjugated (bs-11832R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Cux2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cux2 Polyclonal Antibody, Unconjugated (bs-11832R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Cux2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded Mouse eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cux2 Polyclonal Antibody, Unconjugated (bs-11832R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Cux2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cux2 Polyclonal Antibody, Unconjugated (bs-11832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Product Details

BS-11832R

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:20-1:100	-
ELISA (ELISA)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide derived from human Cux2, amino acids 1001-1085. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 50% glycerol, 1% BSA
Contains	0.09% sodium azide
Storage conditions	-20°C

Target Information

CUX2 may be a transcription factor involved in neural specification. It binds to DNA in a sequence-specific manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cut-like 2; cut-like homeobox 2; Homeobox protein cut-like 2; Homeobox protein Cux-2

Gene Aliases: 1700051K22Rik; CDP2; CUTL2; Cux-2; CUX2; ENSMUSG00000072641; KIAA0293

UniProt ID: (Human) O14529, (Mouse) P70298

Entrez Gene ID: (Human) 23316, (Rat) 288665, (Mouse) 13048

Molecular Function: homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated short-term memory positive regulation of gene expression positive regulation of dendrite morphogenesis cognition positive regulation of synapse assembly positive regulation of dendritic spine morphogenesis cellular response to organic substance positive regulation of excitatory postsynaptic potential transcription from RNA polymerase II promoter negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding sequence-specific DNA binding DNA binding