|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the C-terminus of human cyclin A|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16519 targets Cyclin A in IHC (P), IP, and WB applications and shows reactivity with mouse, Rat, and Human samples.
The PA5-16519 immunogen is a synthetic peptide from the C-terminus of human cyclin A.
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
B-Myb enhances proliferation and suppresses differentiation of keratinocytes in three-dimensional cell culture.
PA5-16519 was used in western blot to study the modulation of keratinocyte proliferation and differentialtion in three-dimensional culture by the Mybl2 transcription factor
|Maruyama H,Ishitsuka Y,Fujisawa Y,Furuta J,Sekido M,Kawachi Y||Archives of dermatological research (306:375)||2014|
Pituitary tumor transforming gene 1 induces tumor necrosis factor-¿ production from keratinocytes: implication for involvement in the pathophysiology of psoriasis.
PA5-16519 was used in immunohistochemistry and western blot to study the induction of keratinocyte TNF-alpha production by the PTTG1 transcription factor and the significance for psoriasis
|Ishitsuka Y,Kawachi Y,Maruyama H,Taguchi S,Fujisawa Y,Furuta J,Nakamura Y,Ishii Y,Otsuka F||The Journal of investigative dermatology (133:2566)||2013|
Expression of cyclin A, cyclin E and p27 in normal, hyperplastic and frankly malignant endometrial samples.
PA5-16519 was used in immunohistochemistry to examine normal, hyperplastic and malignant endometrial tissues for the expression of cyclin A, cyclin E and p27
|Gezginc ST,Celik C,Dogan NU,Toy H,Tazegul A,Colakoglu MC||Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology (33:508)||2013|