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Cyclin B2 Polyclonal Antibody

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Antibody Testing Data (15)

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Cyclin B2 Antibody (21644-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 21644-1-AP) Cyclin B2 antibody at dilution of 1:6,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

Cyclin B2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using CCNB2 antibody 21644-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Cyclin B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP ( Cyclin B2 antibody at dilution of 1:200 (under 40x lens).

Cyclin B2 Antibody in Immunoprecipitation (IP) — IP result of anti-Cyclin B2 (IP:21644-1-AP, 4ug; Detection:21644-1-AP 1:1000) with A431 cells lysate 2000ug.

Product Details

21644-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cyclin B2 Fusion Protein Ag16329 (1-243 aa encoded by BC105086)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MALLRRPTV SSDLENIDTG VNSKVKSHVT IRRTVLEEIG NRVTTRAAQV AKKAQNTKVP VQPTKTTNVN KQLKPTASVK PVQMEKLAPK GPSPTPEDVS MKEENLCQAF SDALLCKIED IDNEDWENPQ LCSDYVKDIY QYLRQLEVLQ SINPHFLDGR DINGRMRAIL VDWLVQVHSK FRLLQETLYM CVGIMDRFLQ VQPVSRKKLQ LVGITALLLA SKYEEMFSPN IEDFVYITDN AYTS (1-243 aa encoded by BC105086)

Target Information

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: G2/mitotic-specific cyclin B2; G2/mitotic-specific cyclin-B2

Gene Aliases: CCNB2; CycB2; HsT17299

UniProt ID: (Human) Q6FI99, (Mouse) P30276

Entrez Gene ID: (Human) 9133, (Rat) 363088, (Mouse) 12442

Molecular Function: kinase activator

Function(s)

G2/M transition of mitotic cell cycle in utero embryonic development mitotic nuclear division mitotic nuclear envelope disassembly growth T cell homeostasis thymus development cell division regulation of cell cycle cell-cell adhesion cell cycle

Process(es)

cyclin-dependent protein serine/threonine kinase activity protein binding cadherin binding involved in cell-cell adhesion