Proteintech

Cyclin B2 Polyclonal Antibody

Product Image Gallery

Cyclin B2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hela cells, using CCNB2 antibody 21644-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Cyclin B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP ( Cyclin B2 antibody at dilution of 1:200 (under 10x lens).

Cyclin B2 Antibody in Western Blot (WB) — rat testis tissue were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cyclin B2 Antibody in Immunoprecipitation (IP) — IP result of anti-Cyclin B2 (IP:21644-1-AP, 4ug; Detection:21644-1-AP 1:1000) with A431 cells lysate 2000ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:3000

View 2 publications 2 publications

Product Specifications

Species

Human, Mouse, Rat

Published species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cyclin B2 Fusion Protein Ag16329

Conjugate

Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.