Proteintech

Cyclin B2 Polyclonal Antibody

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Cyclin B2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using CCNB2 antibody 21644-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Cyclin B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP ( Cyclin B2 antibody at dilution of 1:200 (under 40x lens).

Cyclin B2 Antibody in Western Blot (WB) — rat testis tissue were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cyclin B2 Antibody in Immunoprecipitation (IP) — IP result of anti-Cyclin B2 (IP:21644-1-AP, 4ug; Detection:21644-1-AP 1:1000) with A431 cells lysate 2000ug.

Product Details

21644-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin B2 Fusion Protein Ag16329 (1-243 aa encoded by BC105086) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MALLRRPTV SSDLENIDTG VNSKVKSHVT IRRTVLEEIG NRVTTRAAQV AKKAQNTKVP VQPTKTTNVN KQLKPTASVK PVQMEKLAPK GPSPTPEDVS MKEENLCQAF SDALLCKIED IDNEDWENPQ LCSDYVKDIY QYLRQLEVLQ SINPHFLDGR DINGRMRAIL VDWLVQVHSK FRLLQETLYM CVGIMDRFLQ VQPVSRKKLQ LVGITALLLA SKYEEMFSPN IEDFVYITDN AYTS (1-243 aa encoded by BC105086)

Target Information

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.