Invitrogen

Cyclin B2 Polyclonal Antibody

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Cyclin B2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Cyclin B2 in Hela cells using a Cyclin B2 polyclonal antibody (Product # PA5-97548) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Cyclin B2 Antibody in Western Blot (WB) — Western Blot analysis of Cyclin B2 using a Cyclin B2 Polyclonal antibody (Product # PA5-97548) at a concentration of 2.5 µg/mL. Positive WB detected in: A549 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 46 kDa.

Product Details

PA5-97548

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human G2/mitotic-specific cyclin-B2 protein (7-163AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	4.05 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812164

Target Information

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.