Proteintech

Cyclin H Polyclonal Antibody

View all (16) Cyclin H antibodies

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Cyclin H Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SH-SY5Y cells, using CCNH antibody 10718-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Cyclin H Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cyclin H Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cyclin H Antibody in Flow Cytometry (Flow) — 1X10^6 SH-SY5Y cells were stained with 0.2ug Cyclin H antibody (10718-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:100.

Cyclin H Antibody in Immunoprecipitation (IP) — IP result of anti-Cyclin H (IP:10718-1-AP, 4ug; Detection:10718-1-AP 1:1000) with HeLa cells lysate 1200ug.

Product Details

10718-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin H Fusion Protein Ag1088 (1-323 aa encoded by BC005280) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MYHNSSQKR HWTFSSEEQL ARLRADANRK FRCKAVANGK VLPNDPVFLE PHEEMTLCKY YEKRLLEFCS VFKPAMPRSV VGTACMYFKR FYLNNSVMEY HPRIIMLTCA FLACKVDEFN VSSPQFVGNL RESPLGQEKA LEQILEYELL LIQQLNFHLI VHNPYRPFEG FLIDLKTRYP ILENPEILRK TADDFLNRIA LTDAYLLYTP SQIALTAILS SASRAGITME SYLSESLMLK ENRTCLSQLL DIMKSMRNLV KKYEPPRSEE VAVLKQKLER CHSAELALNV ITKKRKGYED DDYVSKKSKH EEEEWTDDDL VESL (1-323 aa encoded by BC005280)

Target Information

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CAK complex subunit; CDK-activating kinase complex subunit; cyclin-dependent kinase-activating kinase complex subunit; Cyclin-H; MO15-associated protein; OTTHUMP00000222395; OTTHUMP00000222396; p34; p37

Gene Aliases: 6330408H09Rik; AI661354; AV102684; AW538719; CAK; CCNH; CycH; p34; p37

UniProt ID: (Human) P51946, (Mouse) Q61458, (Rat) Q9R1A0

Entrez Gene ID: (Human) 902, (Mouse) 66671, (Rat) 84389

Molecular Function: kinase activator