Search
Invitrogen
Cyp4a10 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 11
Cyp4a10 Antibody (PA3-033) in ICC/IF
Immunofluorescent analysis of Cytochrome P450 4A (green) showing staining in the cytoplasm and membrane of H-4-II-E cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 4A polyclonal antibody (Product # PA3-033) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS... View More
Product Details
PA3-033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat,
Human,
Rabbit,
Rat
Published species
Hamster,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-033 detects cytochrome P450 4A (isoforms 1, 2, & 3) from human, feline, rabbit and rat tissues.
PA3-033 has been successfully used in Western blot, ICC/IF and IHC procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 4A in rat liver extract. Immunohistochemical staining of P450 4A in rat brain with PA3-033 results in perivascular and surrounding cortex staining.
The PA3-033 immunogen is a synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.
Target Information
The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA's which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CYP; CYPIVA10; Cytochrome P450 4A10; cytochrome P450, 4A1; cytochrome P450, 4a10; cytochrome P450, family 4, subfamily a, polypeptide 10; cytochrome P450, family 4, subfamily A, polypeptide 22; cytochrome P450, IVA1; Cytochrome P450-LA-omega 1; Cytochrome P452; Lauric acid omega-hydroxylase; Long-chain fatty acid omega-monooxygenase; P450
Gene Aliases: Cyp4a-1; Cyp4a1; Cyp4a10; Cyp4a22; CYPIVA10
UniProt ID: (Rat) P08516
Entrez Gene ID: (Rat) 50549
monooxygenase activity
iron ion binding
arachidonic acid monooxygenase activity
arachidonic acid epoxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
alkane 1-monooxygenase activity
heme binding
leukotriene-B4 20-monooxygenase activity
arachidonic acid omega-hydroxylase activity
oxygenase
long-chain fatty acid metabolic process
kidney development
placenta development
renal water homeostasis
pressure natriuresis
leukotriene metabolic process
arachidonic acid metabolic process
epoxygenase P450 pathway
positive regulation of icosanoid secretion
leukotriene B4 catabolic process
linoleic acid metabolic process
icosanoid biosynthetic process
lauric acid metabolic process
sodium ion homeostasis
oxidation-reduction process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support