PA3-033 detects cytochrome P450 4A (isoforms 1, 2, and 3) from human, feline, rabbit and rat tissues.
PA3-033 has been successfully used in Western blot, ICC/IF and IHC procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 4A in rat liver extract. Immunohistochemical staining of P450 4A in rat brain with PA3-033 results in perivascular and surrounding cortex staining.
The PA3-033 immunogen is a synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.
The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA's which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, and 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic and brain microvessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CYP; CYPIVA10; Cytochrome P450 4A10; cytochrome P450, 4A1; cytochrome P450, 4a10; cytochrome P450, family 4, subfamily a, polypeptide 10; cytochrome P450, family 4, subfamily A, polypeptide 22; cytochrome P450, IVA1; Cytochrome P450-LA-omega 1; Cytochrome P452; Lauric acid omega-hydroxylase; Long-chain fatty acid omega-monooxygenase; P450
Gene Aliases: Cyp4a-1; Cyp4a1; Cyp4a10; Cyp4a22; CYPIVA10
UniProt ID: (Rat) P08516
Entrez Gene ID: (Rat) 50549