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          • Primary Antibodies ›
          • Cystatin F Antibodies

          Invitrogen

          Cystatin F Polyclonal Antibody

          1 Reference
          View all (10) Cystatin F antibodies

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          Cite Cystatin F Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          Cystatin F Antibody in Immunocytochemistry (ICC/IF)
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          Cystatin F Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Cystatin F Antibody (PA5-103772) in ICC/IF

          Immunocytochemistry analysis of Cystatin F in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Cystatin F polyclonal antibody (Product # PA5-103772) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Cystatin F Antibody in Immunocytochemistry (ICC/IF)
          Cystatin F Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cystatin F Antibody in Western Blot (WB)
          Cystatin F Polyclonal Antibody

          Product Details

          PA5-103772

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:3,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human CST7(Accession O76096), corresponding to amino acid residues R2-A52.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2853105

          Product Specific Information

          Antibody detects endogenous levels of total CST7.

          Target Information

          The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CMAP; CST7; cystatin 7; Cystatin-7; Cystatin-F; Cystatin-like metastasis-associated protein; Leukocystatin

          View more View less

          Gene Aliases: CMAP; CST7

          View more View less

          UniProt ID: (Human) O76096, (Mouse) O89098

          View more View less

          Entrez Gene ID: (Human) 8530, (Mouse) 13011

          View more View less

          Function(s)
          protease binding endopeptidase inhibitor activity cysteine-type endopeptidase inhibitor activity protein binding peptidase inhibitor activity protein homodimerization activity
          Process(es)
          negative regulation of peptidase activity regulation of antigen processing and presentation immune response positive regulation of myelination negative regulation of microglial cell activation lysosomal protein catabolic process
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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