Western blot analysis of skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse skeletal muscle and 4) rat skeletal muscle using a DAAM2 polyclonal antibody (Product # PA5-23201) at 0.5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (116 YAFDEEETEMRNQVVED 132) of DAAM2 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DAAM2 is a recently identified planar cell polarity (PCP) signaling molecule implicated in the regulation of cellular polarity, convergent extension, and invasion. DAAM2 belongs to the formin homology family and contains a DAD (diaphanous autoregulatory) domain, an FH1 (formin homology 1) domain, an FH2 (formin homology 2) domain and a GBD/FH3 (Rho GTPase-binding/ formin homology 3) domain. The DAAM2 protein is known to be involved in cell divison. Though the exact function of DAAM2 is yet unknown, reports suggest that it is closely related to DAAM1 and hence along with DAAM1 it may also be required in Wnt/Fz signaling and activation of Rho and in regulating cytoskeleton architecture. DAAM2 is expressed in most of tissues but the expression level is higher in brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.