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Invitrogen
DACT1 Polyclonal Antibody
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FIGURE: 1 / 2
DACT1 Antibody (PA5-119971) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse cerebellum tissue using DACT1 Polyclonal Antibody (Product # PA5-119971). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the DACT1 antibody at a dilutio... View More
Product Details
PA5-119971
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human DACT1 aa 637-836.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Mouse testis tissue lysate, mouse liver tissue lysate, mouse cerebellum tissue.
Subcellular Location: Cytoplasm, nucleus, synapse.
Target Information
Dapper homolog 1, an ortholog of Xenopus Dpr, antagonizes Wnt signaling by promoting Dvl degradation via a lysosome inhibitor-sensitive and proteasome inhibitor-insensitive mechanism. Dapper homolog 1 acts as an interacting protein for Dvl, and the interaction is formed between the DEP (Dishevelled, Egl-10, and pleckstrin) domain of Dvl and the central and C-terminal regions of Dapper homolog 1. The gene for human Dapper homolog 1 is localized on chromosomal region 14q23.1. The protein impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. The protein is also required for normal notochord formation and is downregulated in hepatocellular carcinomas (HCC). It is expressed in human liver and various other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dapper 1; Dapper antagonist of catenin 1; Dapper homolog 1; dapper, antagonist of beta-catenin, homolog 1; Frodo homolog; hDPR1; Hepatocellular carcinoma novel gene 3 protein; heptacellular carcinoma novel gene 3; HNG3; MDpr1; thymus expressed gene 3; Thymus-expressed novel gene 3 protein; unnamed protein product
Gene Aliases: 4921528D17Rik; AI115603; DACT1; DAPPER; DAPPER1; DPR1; Frd1; FRODO; Frodo1; HDPR1; HNG3; MDpr1; MTNG3; TBS2; THYEX3
UniProt ID: (Human) Q9NYF0, (Mouse) Q8R4A3
Entrez Gene ID: (Human) 51339, (Mouse) 59036
negative regulation of transcription from RNA polymerase II promoter
nervous system development
Wnt signaling pathway
neural tube development
positive regulation of Wnt signaling pathway
negative regulation of Wnt signaling pathway
regulation of protein stability
positive regulation of protein catabolic process
negative regulation of JNK cascade
embryonic hindgut morphogenesis
regulation of canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
regulation of nodal signaling pathway
negative regulation of beta-catenin-TCF complex assembly
regulation of Wnt signaling pathway, planar cell polarity pathway
negative regulation of G1/S transition of mitotic cell cycle
gastrulation with mouth forming second
multicellular organism development
regulation of catenin import into nucleus
positive regulation of catenin import into nucleus
positive regulation of fat cell differentiation
embryonic morphogenesis
dendrite morphogenesis
synapse organization
canonical Wnt signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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