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DACT1 Antibody (PA5-23216) in IHC (P)
Immunohistochemical analysis of DACT1 in formalin-fixed paraffin-embedded human brain. Samples were incubated in DACT1 polyclonal antibody (Product # PA5-23216) using a dilution of 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Product Details
PA5-23216
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus,
Zebrafish
Published species
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 725-775 of human Dapper homolog 1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2540741
Target Information
Dapper homolog 1, an ortholog of Xenopus Dpr, antagonizes Wnt signaling by promoting Dvl degradation via a lysosome inhibitor-sensitive and proteasome inhibitor-insensitive mechanism. Dapper homolog 1 acts as an interacting protein for Dvl, and the interaction is formed between the DEP (Dishevelled, Egl-10, and pleckstrin) domain of Dvl and the central and C-terminal regions of Dapper homolog 1. The gene for human Dapper homolog 1 is localized on chromosomal region 14q23.1. The protein impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. The protein is also required for normal notochord formation and is downregulated in hepatocellular carcinomas (HCC). It is expressed in human liver and various other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dapper 1; Dapper antagonist of catenin 1; Dapper homolog 1; dapper homolog 1, antagonist of beta-catenin; dapper, antagonist of beta-catenin; dapper, antagonist of beta-catenin, homolog 1; dapper1b; dishevelled-binding antagonist of beta-catenin 1; dishevelled-interacting protein; Frodo; frodo 1; Frodo homolog; functional regulator of dsh in ontogenesis; hDPR1; Hepatocellular carcinoma novel gene 3 protein; heptacellular carcinoma novel gene 3; MDpr1; signaling protein; thymus expressed gene 3; Thymus-expressed novel gene 3 protein
Gene Aliases: 4921528D17Rik; AI115603; DACT1; dact1-a; dact1-b; DAPPER; DAPPER1; dpr; DPR1; Frd1; FRODO; Frodo1; HDPR1; HNG3; MDpr1; MTNG3; RGD1564008; THYEX3; XDpr; XDpr1b; XELAEV_18041122mg
UniProt ID: (Human) Q9NYF0, (Mouse) Q8R4A3
Entrez Gene ID: (Human) 51339, (Chicken) 723789, (Rat) 500666, (Mouse) 59036, (Zebrafish) 405799, (Xenopus) 398315
multicellular organism development
Wnt signaling pathway
negative regulation of JNK cascade
negative regulation of canonical Wnt signaling pathway
regulation of Wnt signaling pathway, planar cell polarity pathway
negative regulation of transcription from RNA polymerase II promoter
neural tube development
positive regulation of Wnt signaling pathway
negative regulation of Wnt signaling pathway
regulation of protein stability
negative regulation of protein binding
positive regulation of protein binding
regulation of catenin import into nucleus
positive regulation of catenin import into nucleus
positive regulation of protein catabolic process
embryonic hindgut morphogenesis
regulation of nodal signaling pathway
positive regulation of cellular protein catabolic process
negative regulation of beta-catenin-TCF complex assembly
negative regulation of G1/S transition of mitotic cell cycle
gastrulation with mouth forming second
positive regulation of fat cell differentiation
embryonic morphogenesis
dendrite morphogenesis
synapse organization
canonical Wnt signaling pathway
nervous system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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