|Tested species reactivity||C. elegans|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 700-796 of the C.elegans DAF-3 protein.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Signals from TGF-beta superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans (C.elegans), defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. DAF-3 transgenes interfere with dauer formation, playing a role in the nucleus. DAF-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.