Invitrogen

DAGLA Polyclonal Antibody

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Antibody Testing Data (3)

DAGLA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DAGLA Antibody (PA5-23765) in IHC (P)

Immunohistochemistry analysis of DAGLA in formalin fixed and paraffin embedded human brain tissue. Samples were incubated with DAGLA polyclonal antibody (Product # PA5-23765) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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Product Details

PA5-23765

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 317-345 amino acids from the central region of human DAGLA

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2541265

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Target Information

This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endocannabinoid dysfunction in neurological disease: neuro-ocular DAGLA-related syndrome.

Brain : a journal of neurology

Bainbridge MN,Mazumder A,Ogasawara D,Abou Jamra R,Bernard G,Bertini E,Burglen L,Cope H,Crawford A,Derksen A,Dure L,Gantz E,Koch-Hogrebe M,Hurst ACE,Mahida S,Marshall P,Micalizzi A,Novelli A,Peng H,Rodriguez D,Robbins SL,Rutledge SL,Scalise R,Schließke S,Shashi V,Srivastava S,Thiffault I,Topol S,Qebibo L,Wieczorek D,Cravatt B,Haricharan S,Torkamani A,Friedman J

PA5-23765 was used in Immunocytochemistry to identify nine children from eight families with heterozygous, de novo truncating variants in the last exon of DAGLA with a neuro-ocular phenotype characterized by developmental delay, ataxia and complex oculomotor abnormality.

Fri Oct 21 00:00:00 EDT 2022

The TRPV1 Receptor Is Up-Regulated by Sphingosine 1-Phosphate and Is Implicated in the Anandamide-Dependent Regulation of Mitochondrial Activity in C2C12 Myoblasts.

International journal of molecular sciences

Standoli S,Pecchioli S,Tortolani D,Di Meo C,Fanti F,Sergi M,Bacci M,Seidita I,Bernacchioni C,Donati C,Bruni P,Maccarrone M,Rapino C,Cencetti F

PA5-23765 was used in Western Blot to use C2C12 myoblasts to investigate the effects of sphingosine 1-phosphate (S1P) on endocannabinoid (ECS) elements to study the interactions between S1P and ECS in skeletal muscle.

Wed Sep 21 00:00:00 EDT 2022

Involvement of Plasma Endocannabinoids and the Hypothalamic Endocannabinoid System in Increasing Feed Intake after Parturition of Dairy Cows.

Neuroendocrinology

Kuhla B,Kaever V,Tuchscherer A,Kuhla A

PA5-23765 was used in Immunohistochemistry to show a potential involvement of AEA in stimulating feed intake and of 2-AG in regulating energy metabolism of early lactating cows.

Mon Feb 15 00:00:00 EST 2021

Bioinformatics

Protein Aliases: DAGL-alpha; DGL-alpha; Diacylglycerol lipase-alpha; Neural stem cell-derived dendrite regulator; Sn1-specific diacylglycerol lipase alpha

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Gene Aliases: C11orf11; DAGL(ALPHA); DAGLA; DAGLALPHA; KIAA0659; NSDDR

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UniProt ID: (Human) Q9Y4D2, (Mouse) Q6WQJ1

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Entrez Gene ID: (Human) 747, (Mouse) 269060

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Function(s)

metal ion binding acylglycerol lipase activity phospholipase activity prenylcysteine methylesterase activity lipase activity hydrolase activity 1-oxa-2-oxocycloheptane lactonase activity sulfolactone hydrolase activity 3,4-dihydrocoumarin hydrolase activity butyrolactone hydrolase activity endosulfan lactone lactonase activity L-ascorbate 6-phosphate lactonase activity Ser-tRNA(Thr) hydrolase activity Ala-tRNA(Pro) hydrolase activity Cys-tRNA(Pro) hydrolase activity Ser(Gly)-tRNA(Ala) hydrolase activity all-trans-retinyl-palmitate hydrolase, all-trans-retinol forming activity retinyl-palmitate esterase activity mannosyl-oligosaccharide 1,6-alpha-mannosidase activity mannosyl-oligosaccharide 1,3-alpha-mannosidase activity methyl indole-3-acetate esterase activity methyl salicylate esterase activity methyl jasmonate esterase activity hydrolase lipase metabolite interconversion enzyme

Process(es)

neuroblast proliferation arachidonic acid metabolic process neurotransmitter biosynthetic process diacylglycerol catabolic process endocannabinoid signaling pathway lipid metabolic process lipid catabolic process neurogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DAGLA Polyclonal Antibody

DAGLA Antibody (PA5-23765) in IHC (P)

Product Details

PA5-23765

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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