Invitrogen

DAK Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (9) DAK antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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DAK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of DAK in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a DAK Polyclonal Antibody (Product # PA5-58810).

DAK Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of DAK in human duodenum, kidney, liver and skeletal muscle using DAK Polyclonal Antibody (Product # PA5-58810) (A) shows similar protein distribution across tissues to an independent DAK Polyclonal Antibody (B).

DAK Antibody in Western Blot (WB) — Western blot analysis of DAK in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a DAK Polyclonal Antibody (Product # PA5-58810).

DAK Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of DAK demonstrates antibody specificity. Immunohistochemical analysis of DAK using anti-DAK Polyclonal Antibody (Product # PA5-58810), shows significant staining of DAK in human duodenum and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of DAK within each tissue is shown using RNA-Seq.

Product Details

PA5-58810

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:5,000-1:10,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DAK

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2640373

Product Specific Information

Immunogen sequence: SVPGSKPTFE LSADEVELGL GIHGEAGVRR IKMATADEIV KLMLDHMTNT TNASHVPVQP GSSVVMMVNN LGGLSFLELG IIADATVRSL EGRGVKIARA

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 81%.

Target Information

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent dihydroxyacetone kinase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); DHA kinase; Dha kinase/FMN cyclase; dihydroxyacetone kinase 2 homolog; DKFZP586B1621; FAD-AMP lyase (cyclic FMN forming); FAD-AMP lyase (cyclizing); FAD-AMP lyase cyclic FMN forming; FAD-AMP lyase cyclizing; FMN cyclase; Glycerone kinase; MGC5621; testis tissue sperm-binding protein Li 84P; Triokinase; Triokinase/FMN cyclase; Triose kinase

Gene Aliases: DAK; NET45; TKFC

UniProt ID: (Human) Q9H895

Entrez Gene ID: (Human) 26007

Molecular Function: cyclase

Function(s)

glycerol metabolic process negative regulation of MDA-5 signaling pathway cellular carbohydrate metabolic process innate immune response regulation of innate immune response carbohydrate phosphorylation fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate

Process(es)

glycerone kinase activity protein binding ATP binding FAD-AMP lyase (cyclizing) activity metal ion binding triokinase activity