Invitrogen

DAK Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (9) DAK antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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DAK Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of DAK in human duodenum, kidney, liver and skeletal muscle using DAK Polyclonal Antibody (Product # PA5-61682) (A) shows similar protein distribution across tissues to an independent DAK Polyclonal Antibody (B).

DAK Antibody in Western Blot (WB) — Western blot analysis of DAK in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG; Lane 4: Human plasma; Lane 5: Human liver tissue. Samples were probed using a DAK Polyclonal Antibody (Product # PA5-61682).

DAK Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of DAK demonstrates antibody specificity. Immunohistochemical analysis of DAK using anti-DAK Polyclonal Antibody (Product # PA5-61682), shows significant staining of DAK in human duodenum and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of DAK within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-61682

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human DAK View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2640374

Product Specific Information

Immunogen sequence: PGDRTMLDSL WAAGQELQAW KSPGADLLQV LTKAVKSAEA AAEATKNMEA GAGRASYISS ARLEQPDPGA VAAAAILRAI L

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 93%.

Target Information

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent dihydroxyacetone kinase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); DHA kinase; Dha kinase/FMN cyclase; dihydroxyacetone kinase 2 homolog; DKFZP586B1621; FAD-AMP lyase (cyclic FMN forming); FAD-AMP lyase (cyclizing); FAD-AMP lyase cyclic FMN forming; FAD-AMP lyase cyclizing; FMN cyclase; Glycerone kinase; MGC5621; testis tissue sperm-binding protein Li 84P; Triokinase; Triokinase/FMN cyclase; Triose kinase

Gene Aliases: DAK; NET45; TKFC

UniProt ID: (Human) Q3LXA3

Entrez Gene ID: (Human) 26007

Molecular Function: cyclase