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Invitrogen
DAP Polyclonal Antibody
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FIGURE: 1 / 8
DAP Antibody (PA5-106935) in ICC/IF
Immunocytochemistry analysis of DAP in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with DAP polyclonal antibody (Product # PA5-106935) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106935
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DAP(Accession P51397), corresponding to amino acid residues M1-K37.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total DAP.
Target Information
This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: amylin peptide; DAP 1; DAP-1; Death-associated protein 1; MGC99796; OTTHUMP00000161670; OTTHUMP00000221331; Rap7a
Gene Aliases: 4921531N22Rik; AI789753; AU040360; DAP; DAP1; Rap7a
UniProt ID: (Human) P51397, (Mouse) Q91XC8, (Rat) Q9QX67
Entrez Gene ID: (Human) 1611, (Mouse) 223453, (Rat) 64322
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of autophagy
negative regulation of NF-kappaB transcription factor activity
cellular response to amino acid starvation
negative regulation of transcription, DNA-templated
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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