|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Gly903 of human DAPK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
It is not recommended to aliquot this antibody.
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DAPK1-p53 interaction converges necrotic and apoptotic pathways of ischemic neuronal death.
PA5-17055 was used in immunoprecipitation to study the interaction between DAPK1 and p53 as the point of convergence of the apoptotic and necrotic pathways involved in ischemic neuronal cell death
|Pei L,Shang Y,Jin H,Wang S,Wei N,Yan H,Wu Y,Yao C,Wang X,Zhu LQ,Lu Y||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:6546)||2014|