Invitrogen

DARPP-32 Polyclonal Antibody

Product Image Gallery

DARPP-32 Antibody in Western Blot (WB) — Western blot of a rat hippocampal lysate showing the specific immunolabeling of the ~32 kDa DARPP protein.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:250

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the N-terminal region of rat PPP1R1B conjugated to KLH

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_558596

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on 100% sequence homology.

This antibody is specific for the ~32 kDa DARPP-32 protein.

Target Information

DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; neuronal phosphoprotein DARPP-32; Protein phosphatase 1 regulatory subunit 1B

Gene Aliases: Darpp-32; Darpp32; Ppp1r1b

UniProt ID: (Rat) Q6J4I0

Entrez Gene ID: (Rat) 360616

Molecular Function: enzyme modulator phosphatase inhibitor phosphatase modulator signaling molecule