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Rockland
DAXX Polyclonal Antibody
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FIGURE: 1 / 1
DAXX Antibody (600-401-966) in WB
Western blot using Rocklands affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25,000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.
Product Details
600-401-966
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-275 of human DAXX protein.
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human DAXX protein. A BLAST analysis was used to suggest cross reactivity with DAXX protein from human, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP-C binding protein; DADB-159G18.9; Daxx; Death domain-associated protein 6; death-associated protein 6; death-domain associated protein; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; hDaxx; MGC126245; MGC126246; SMIM40
Gene Aliases: BING2; DAP6; DAXX; EAP1
UniProt ID: (Human) Q9UER7, (Mouse) O35613
Entrez Gene ID: (Human) 1616, (Mouse) 13163, (Rat) 140926
p53 binding
transcription corepressor activity
receptor signaling protein activity
protein binding
transcription factor binding
enzyme binding
protein kinase binding
protein kinase activator activity
heat shock protein binding
ubiquitin protein ligase binding
histone binding
protein N-terminus binding
androgen receptor binding
transcription coactivator activity
protein homodimerization activity
general transcription factor
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
nucleosome assembly
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
activation of JUN kinase activity
extrinsic apoptotic signaling pathway via death domain receptors
response to metal ion
negative regulation of myotube differentiation
viral process
covalent chromatin modification
androgen receptor signaling pathway
PML body organization
regulation of protein ubiquitination
positive regulation of histone phosphorylation
positive regulation of apoptotic process
positive regulation of protein kinase activity
negative regulation of transcription, DNA-templated
cellular response to tumor necrosis factor
cellular response to anoxia
positive regulation of neuron death
mitotic cytokinesis
chromatin modification
positive regulation of transcription from RNA polymerase II promoter
apoptotic signaling pathway
negative regulation of nucleic acid-templated transcription
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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