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DAZAP2 Antibody (PA5-98445) in ICC/IF
Immunofluorescent analysis of DAZAP2 in MCF-7 cells using a DAZAP2 polyclonal antibody (Product # PA5-98445) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-98445
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human DAZ-associated protein 2 protein (1-168AA)
Conjugate
Form
Liquid
Concentration
4.32 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2813058
Target Information
DAZAP2 encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAZ-associated protein 2; deleted in azoospermia associated protein 2; Deleted in azoospermia-associated protein 2; proline-rich transcript in brain; Proline-rich transcript in brain protein; proline-rich transcript, brain-expressed protein
Gene Aliases: DAZAP2; KIAA0058; PRTB
UniProt ID: (Human) Q15038
Entrez Gene ID: (Human) 9802, (Rat) 300235
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