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DBR1 Polyclonal Antibody

View all (7) DBR1 antibodies

Questions & Answers

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Questions & Answers

Tech Support

Antibody Testing Data (6)

DBR1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

DBR1 Antibody (16019-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using DBR1 antibody 16019-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Product Image Gallery

DBR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using DBR1 antibody 16019-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

DBR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens).

DBR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens).

DBR1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DBR1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DBR1 Antibody in Western Blot (WB) — WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

Product Details

16019-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DBR1 Fusion Protein Ag8830 (204-544 aa encoded by BC009472)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: TLGSPAASE LLEHLKPTYW FSAHLHVKFA ALMQHQAKDK GQTARATKFL ALDKCLPHRD FLQILEIEHD PSAPDYLEYD IEWLTILRAT DDLINVTGRL WNMPENNGLH ARWDYSATEE GMKEVLEKLN HDLKVPCNFS VTAACYDPSK PQTQMQLIHR INPQTTEFCA QLGIIDINVR LQKSKEEHHV CGEYEEQDDV ESNDSGEDQS EYNTDTSALS SINPDEIMLD EEEDEDSIVS AHSGMNTPSV EPSDQASEFS ASFSDVRILP GSMIVSSDDT VDSTIDREGK PGGTVESGNG EDLTKVPLKR LSDEHEPEQR KKIKRRNQAI YAAVDDDDDD AA (204-544 aa encoded by BC009472)

Target Information

The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous Cell Carcinoma.

Technology in cancer research & treatment

Xu X,Yang X,Liu X,Bi Y,Kong P,Wang Y,Cheng X,Xi Y

Mon May 02 00:00:00 EDT 2022

Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.

Cell

Zhang SY,Clark NE,Freije CA,Pauwels E,Taggart AJ,Okada S,Mandel H,Garcia P,Ciancanelli MJ,Biran A,Lafaille FG,Tsumura M,Cobat A,Luo J,Volpi S,Zimmer B,Sakata S,Dinis A,Ohara O,Garcia Reino EJ,Dobbs K,Hasek M,Holloway SP,McCammon K,Hussong SA,DeRosa N,Van Skike CE,Katolik A,Lorenzo L,Hyodo M,Faria E,Halwani R,Fukuhara R,Smith GA,Galvan V,Damha MJ,Al-Muhsen S,Itan Y,Boeke JD,Notarangelo LD,Studer L,Kobayashi M,Diogo L,Fairbrother WG,Abel L,Rosenberg BR,Hart PJ,Etzioni A,Casanova JL

Thu Feb 22 00:00:00 EST 2018

Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.

Cell

Zhang SY,Clark NE,Freije CA,Pauwels E,Taggart AJ,Okada S,Mandel H,Garcia P,Ciancanelli MJ,Biran A,Lafaille FG,Tsumura M,Cobat A,Luo J,Volpi S,Zimmer B,Sakata S,Dinis A,Ohara O,Garcia Reino EJ,Dobbs K,Hasek M,Holloway SP,McCammon K,Hussong SA,DeRosa N,Van Skike CE,Katolik A,Lorenzo L,Hyodo M,Faria E,Halwani R,Fukuhara R,Smith GA,Galvan V,Damha MJ,Al-Muhsen S,Itan Y,Boeke JD,Notarangelo LD,Studer L,Kobayashi M,Diogo L,Fairbrother WG,Abel L,Rosenberg BR,Hart PJ,Etzioni A,Casanova JL

Thu Feb 22 00:00:00 EST 2018

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

International journal of molecular sciences

Masaki S,Yoshimoto R,Kaida D,Hata A,Satoh T,Ohno M,Kataoka N

Mon Feb 09 00:00:00 EST 2015

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

International journal of molecular sciences

Masaki S,Yoshimoto R,Kaida D,Hata A,Satoh T,Ohno M,Kataoka N

Mon Feb 09 00:00:00 EST 2015

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

International journal of molecular sciences

Masaki S,Yoshimoto R,Kaida D,Hata A,Satoh T,Ohno M,Kataoka N

Mon Feb 09 00:00:00 EST 2015

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

International journal of molecular sciences

Masaki S,Yoshimoto R,Kaida D,Hata A,Satoh T,Ohno M,Kataoka N

Mon Feb 09 00:00:00 EST 2015

Inhibition of U4 snRNA in human cells causes the stable retention of polyadenylated pre-mRNA in the nucleus.

PloS one

Hett A,West S

Fri Jun 12 00:00:00 EDT 2015

Inhibition of U4 snRNA in human cells causes the stable retention of polyadenylated pre-mRNA in the nucleus.

PloS one

Hett A,West S

Fri Jun 12 00:00:00 EDT 2015

Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.

Molecular & cellular proteomics : MCP

Syred HM,Welburn J,Rappsilber J,Ohkura H

Fri Nov 01 00:00:00 EDT 2013

Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.

Molecular & cellular proteomics : MCP

Syred HM,Welburn J,Rappsilber J,Ohkura H

Fri Nov 01 00:00:00 EDT 2013

Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.

Nature genetics

Armakola M,Higgins MJ,Figley MD,Barmada SJ,Scarborough EA,Diaz Z,Fang X,Shorter J,Krogan NJ,Finkbeiner S,Farese RV,Gitler AD

Sat Dec 01 00:00:00 EST 2012

Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.

Nature genetics

Armakola M,Higgins MJ,Figley MD,Barmada SJ,Scarborough EA,Diaz Z,Fang X,Shorter J,Krogan NJ,Finkbeiner S,Farese RV,Gitler AD

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: debranching enzyme homolog 1; Lariat debranching enzyme; RNA lariat debranching enzyme

Gene Aliases: DBR1

UniProt ID: (Human) Q9UK59

Entrez Gene ID: (Human) 51163

Molecular Function: endoribonuclease

RNA splicing, via transesterification reactions mRNA splicing, via spliceosome RNA phosphodiester bond hydrolysis, endonucleolytic

Process(es)

RNA lariat debranching enzyme activity poly(A) RNA binding metal ion binding