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Invitrogen
DCP1A Polyclonal Antibody
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FIGURE: 1 / 3
DCP1A Antibody (PA5-75261) in ICC/IF
Immunofluorescence analysis of DCP1A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with DCP1A Polyclonal Antibody(Product # PA5-75261) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (... View More
Product Details
PA5-75261
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 102-150 of Human DCP1A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DCP1 decapping enzyme homolog A; DCP1 decapping enzyme homolog A (S. cerevisiae) isoform DCP1A_1; DCP1 decapping enzyme homolog A (S. cerevisiae) isoform DCP1A_3; DCP1 decapping enzyme homolog A (S. cerevisiae) isoform DCP1A_4; DCP1 decapping enzyme-like protein A; decapping enzyme; decapping enzyme hDcp1a; FLJ21691; MAD homolog 4 interacting transcription coactivator 1; MAD homolog 4-interacting transcription coactivator 1; mRNA-decapping enzyme 1A; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator; test2; Transcription factor SMIF; unnamed protein product
Gene Aliases: 1110066A22Rik; 4930568L04Rik; AU019772; D14Ertd817e; DCP1A; HSA275986; Mitc1; Nbla00360; RGD1561298; SMAD4IP1; SMIF
UniProt ID: (Human) Q9NPI6, (Mouse) Q91YD3
Entrez Gene ID: (Human) 55802, (Rat) 361109, (Mouse) 75901
mRNA binding
protein binding
enzyme activator activity
hydrolase activity
kinesin binding
identical protein binding
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity
RNA 7-methylguanosine cap binding
enzyme regulator activity
GPI-anchor transamidase activity
transcription factor binding
coenzyme F390-A hydrolase activity
coenzyme F390-G hydrolase activity
mRNA capping factor
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
deadenylation-dependent decapping of nuclear-transcribed mRNA
deadenylation-independent decapping of nuclear-transcribed mRNA
mRNA methylguanosine-cap decapping
protein localization to cytoplasmic stress granule
regulation of catalytic activity
transforming growth factor beta receptor signaling pathway
positive regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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