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Agrisera
DCP5/Protein decapping 5 Polyclonal Antibody
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FIGURE: 1 / 2
DCP5/Protein decapping 5 Antibody (AS23-4944) in WB
Samples: 1 - HA-DCP 1 (transient in N. Benthamiana) 2 - HA-DCP 2 (transient in N. Benthamiana) 3 - HA-DCP 5 (transient in N. Benthamiana) Total protein extracted freshly from 2 leaf discs (Ø6 mm) of 4-week-old Nicotiana benthamiana or Arabidopsis thaliana leaves in 150 µL extraction buffer (100 m M Tris p H=7.5,1 m M EDTA, 3% SDS) + 50 µL 4 X LaemmLi buffer (Bio-Rad) and denatured at 95°C for 10 min. Sample... View More
Product Details
AS23-4944
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana DCP5.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Nicotiana benthamiana (transiently expressed)
Target Information
DCP5 encodes a cytoplasmic RNA granule protein that concentrates in discrete processing bodies (P-bodies), where it colocalizes and physically associates with core mRNA decapping factors including DCP1 and the catalytic decapping enzyme DCP2. The DCP5 protein is built around an N-terminal Lsm domain followed by a Ser/Thr-rich low-complexity region and a C-terminal segment containing two RGG-rich regions separated by an FDF motif, a modular architecture consistent with RNA/protein interactions and ribonucleoprotein condensate assembly. Functionally, genetic and biochemical data support DCP5 as an mRNA decapping activator and P-body nucleator required for efficient decapping in vivo (without directly stimulating DCP2 catalysis in vitro), and as a translational repressor that helps keep selected mRNAs (including seed storage protein transcripts during germination) translationally silent and routed toward decay; more recently, DCP5 has also been shown to participate in transcriptional control of FLOWERING LOCUS C in a mechanism involving phase-separation behavior mediated by prion-like features.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT1G26110; F14G11.8; F14G11_8; F28B23.21
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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