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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • DDAH2 Antibodies

          Invitrogen

          DDAH2 Polyclonal Antibody

          View all (13) DDAH2 antibodies

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          Cite DDAH2 Polyclonal Antibody

          • Antibody Testing Data (3)
          DDAH2 Antibody in Immunocytochemistry (ICC/IF)
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          DDAH2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          DDAH2 Antibody (PA5-17996) in ICC/IF

          Immunocytochemistry analysis of DDAH2 using DDAH2 Polyclonal Antibody (Product # PA5-17996) in paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DDAH2 Antibody in Immunocytochemistry (ICC/IF)
          DDAH2 Antibody in Western Blot (WB)
          DDAH2 Antibody in Flow Cytometry (Flow)
          DDAH2 Polyclonal Antibody

          Product Details

          PA5-17996

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.3 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          -

          Flow Cytometry (Flow)

          10 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide sequence (SSLCLVLSTRPHS) corresponding to the C-terminus amino acids of DDAH2 (aa 273-285).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          TBS, pH 7.3, with 0.5% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10986709

          Product Specific Information

          This antibody is predicted to react with bovine, canine and rat based on sequence homology.

          This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

          Target Information

          This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: DADB-110M10.5; DDAH-2; DDAHII; dimethylargininase-2; dimethylarginine dimethylaminohydrolase II; epididymis secretory protein Li 277; Inactive N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; OTTHUMP00000029307; OTTHUMP00000062666; OTTHUMP00000174406; Protein G6a; Putative hydrolase DDAH2; S-phase protein; testis tissue sperm-binding protein Li 54e

          View more View less

          Gene Aliases: DDAH; DDAH2; DDAHII; G6A; HEL-S-277; NG30

          View more View less

          UniProt ID: (Human) O95865

          View more View less

          Entrez Gene ID: (Human) 23564

          View more View less

          Function(s)
          catalytic activity protein binding dimethylargininase activity amino acid binding hydrolase
          Process(es)
          citrulline metabolic process arginine catabolic process nitric oxide biosynthetic process nitric oxide mediated signal transduction negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process regulation of nitric-oxide synthase activity
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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