Immunofluorescent analysis of DDAH2 showing staining in the mitochondria of SKNSH cells. SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 15 min and stained using a DDAH2 polyclonal antibody (Product # PA5-30684) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 65 and 285 of Human DDAH2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30684 targets DDAH2 in WB applications and shows reactivity with Human samples.
The PA5-30684 immunogen is recombinant fragment corresponding to a region within amino acids 65 and 285 of Human DDAH2.
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.