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Invitrogen
DDB1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Details
PA5-21282
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 749 and 1140 of DDB1 (Uniprot ID#Q16531)
Conjugate
Form
Liquid
Concentration
0.81 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal, 0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11157449
Product Specific Information
Recommended positive controls: 293T, HepG2, NIH-3T3, mouse brain, PC-12, Rat2, rat brain.
Predicted reactivity: Mouse (99%), Rat (100%), Xenopus laevis (96%), Xenopus Tropicalis (93%), Chicken (98%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: damage specific DNA binding protein 1; damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE-BF; XPE-binding factor
Gene Aliases: 127kDa; AA408517; DDB1; DDBA; p127-Ddb1; UV-DDB1; XAP1; XPCE; XPE; XPE-BF
UniProt ID: (Human) Q16531, (Mouse) Q3U1J4
Entrez Gene ID: (Human) 1642, (Mouse) 13194, (Rat) 64470
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
viral process
Wnt signaling pathway
nucleotide-excision repair, DNA incision
histone H2A monoubiquitination
DNA damage response, detection of DNA damage
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of viral genome replication
positive regulation by virus of viral protein levels in host cell
interaction with symbiont
global genome nucleotide-excision repair
UV-damage excision repair
regulation of mitotic cell cycle phase transition
positive regulation of viral release from host cell
cellular response to DNA damage stimulus
protein ubiquitination
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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