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Invitrogen
DDB1 Polyclonal Antibody
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FIGURE: 1 / 5
DDB1 Antibody (PA5-27113) in ICC/IF
DDB1 Polyclonal Antibody [N1N3] detects DDB1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDB1 protein stained by DDB1 Polyclonal Antibody [N1N3] (Product # PA5-27113) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-27113
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human DDB1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, HeLaS3, Raji, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (96%), Chicken (99%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; unnamed protein product; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE-BF; XPE-binding factor
Gene Aliases: 127kDa; AA408517; DDB1; DDBA; p127-Ddb1; UV-DDB1; WHIKERS; XAP1; XPCE; XPE; XPE-BF
UniProt ID: (Human) Q16531, (Mouse) Q3U1J4
Entrez Gene ID: (Human) 1642, (Mouse) 13194
DNA repair
nucleotide-excision repair
ubiquitin-dependent protein catabolic process
apoptotic process
cellular response to DNA damage stimulus
spindle assembly involved in female meiosis
proteasomal protein catabolic process
Wnt signaling pathway
protein ubiquitination
viral release from host cell
cellular response to UV
ectopic germ cell programmed cell death
regulation of circadian rhythm
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
epigenetic programming in the zygotic pronuclei
positive regulation of viral genome replication
positive regulation of gluconeogenesis
positive regulation of protein catabolic process
positive regulation by virus of viral protein levels in host cell
rhythmic process
negative regulation of developmental process
interaction with symbiont
negative regulation of protein kinase B signaling
type I interferon signaling pathway
UV-damage excision repair
base-excision repair, AP site formation via deaminated base removal
regulation of mitotic cell cycle phase transition
negative regulation of reproductive process
histone H2A monoubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of viral release from host cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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