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Invitrogen
DDB2 Polyclonal Antibody
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FIGURE: 1 / 8
DDB2 Antibody (PA5-79143) in ICC/IF
Immunocytochemistry analysis of DDB2 using anti-DDB2 antibody (Product # PA5-79143). DDB2 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DDB2 antibody (Product # PA5-79143)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visuali... View More
Product Details
PA5-79143
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DDB2 recombinant protein (Position: M1-T115).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A431 whole cell, SW620 whole cell, HELA whole cell, JURKAT whole cell. ICC/IF: U20S cell. Flow: 293T cell.
Target Information
Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDBb; DNA damage-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2; xeroderma pigmentosum group E protein
Gene Aliases: DDB2; DDBB; UV-DDB2; XPE
UniProt ID: (Human) Q92466
Entrez Gene ID: (Human) 1643
protein polyubiquitination
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
DNA repair
nucleotide-excision repair
pyrimidine dimer repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
response to UV
nucleotide-excision repair, DNA incision
histone H2A monoubiquitination
protein autoubiquitination
global genome nucleotide-excision repair
UV-damage excision repair
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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