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          • Primary Antibodies ›
          • DYKDDDDK Tag Antibodies

          Proteintech

          DDDDK Tag Polyclonal Antibody

          861 References
          View all (92) DYKDDDDK Tag antibodies

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          Cite DDDDK Tag Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          DDDDK Tag Antibody in Immunocytochemistry (ICC/IF)
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          DDDDK Tag Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          DDDDK Tag Antibody (20543-1-AP) in ICC/IF

          Immunofluorescent analysis of Transfected HEK-293 cells using 20543-1-AP (Flag tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          DDDDK Tag Antibody in Immunocytochemistry (ICC/IF)
          DDDDK Tag Antibody in Western Blot (WB)
          DDDDK Tag Antibody in Western Blot (WB)
          DDDDK Tag Antibody in Western Blot (WB)

          Product Details

          20543-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:4,000
          View 421 publications 421 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 63 publications 63 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 6 publications 6 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 201 publications 201 publications

          ChIP assay (ChIP)

          -
          View 5 publications 5 publications

          RNA Immunoprecipitation (RIP)

          -
          View 7 publications 7 publications

          Miscellaneous PubMed (Misc)

          -
          View 23 publications 23 publications

          Not applicable (N/A)

          -
          View 134 publications 134 publications
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Human, Mouse, Non-human primate, Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          DDDDK Tag Fusion Protein Ag2329

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.24 mg/mL

          Amount

          36 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is a rabbit polyclonal antibody raised against the 3xDYKDDDDKT sequence and recognizes the (1x) and (3x) DYKDDDDK peptide and detects DDDDK-tagged proteins.

          Target Information

          DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

          View more View less

          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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