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DDR2 Antibody (PA5-95551) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of DDR2 in U87 cells using DDR2 Polyclonal Antibody (Product # PA5-95551). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualized using a fluorescence microscope and filter sets appropriate for the label used.
Product Details
PA5-95551
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DDR2 recombinant protein (Position: T801-E855).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807353
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HT1080 whole cell, human K562 whole cell, human Hela whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. ICC/IF: U87 cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
DDR2 is a member of a subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. DDR1 and DDR2 have been shown to be potently inhibited by Gleevec. DDR2 required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD167 antigen-like family member B; CD167b; CD167b antigen; CD_antigen: CD167b; cell migration-inducing protein 20; Discoidin domain receptor 2; discoidin domain receptor family, member 2; Discoidin domain-containing receptor 2; Discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; RP11-572K18.1; tyrosine-protein kinase TYRO 10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase
Gene Aliases: AW495251; DDR2; MIG20a; NTRKR3; TKT; TYRO10
UniProt ID: (Human) Q16832, (Mouse) Q62371
Entrez Gene ID: (Human) 4921, (Mouse) 18214, (Rat) 685781
transmembrane receptor protein tyrosine kinase activity
protein binding
collagen binding
ATP binding
protein tyrosine kinase collagen receptor activity
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
ossification
endochondral bone growth
cell adhesion
signal transduction
regulation of extracellular matrix disassembly
positive regulation of fibroblast migration
peptidyl-tyrosine phosphorylation
extracellular matrix organization
collagen fibril organization
regulation of bone mineralization
biomineral tissue development
chondrocyte proliferation
collagen-activated tyrosine kinase receptor signaling pathway
positive regulation of osteoblast differentiation
positive regulation of protein kinase activity
protein autophosphorylation
positive regulation of fibroblast proliferation
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of extracellular matrix disassembly
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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