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DDX1 Polyclonal Antibody

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DDX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using (Product # 11357-1-AP) (DDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DDX1 Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 11357-1-AP (DDX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDX1 Antibody in Immunoprecipitation (IP) — IP result of anti-DDX1 (IP:11357-1-AP, 3ug; Detection:11357-1-AP 1:1000) with PC-3 cells lysate 5000ug.

Product Details

11357-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DDX1 Fusion Protein Ag1887 (391-740 aa encoded by BC012132) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.39 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: IPQVTSDGK RLQVIVCSAT LHSFDVKKLS EKIMHFPTWV DLKGEDSVPD TVHHVVVPVN PKTDRLWERL GKSHIRTDDV HAKDNTRPGA NSPEMWSEAI KILKGEYAVR AIKEHKMDQA IIFCRTKIDC DNLEQYFIQQ GGGPDKKGHQ FSCVCLHGDR KPHERKQNLE RFKKGDVRFL ICTDVAARGI DIHGVPYVIN VTLPDEKQNY VHRIGRVGRA ERMGLAISLV ATEKEKVWYH VCSSRGKGCY NTRLKEDGGC TIWYNEMQLL SEIEEHLNCT ISQVEPDIKV PVDEFDGKVT YGQKRAAGGG SYKGHVDILA PTVQELAALE KEAQTSFLHL GYLPNQLFRT F (391-740 aa encoded by BC012132)

Target Information

DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Nucleic acids research

Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

Fri Dec 14 00:00:00 EST 2018

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Nucleic acids research

Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

Fri Dec 14 00:00:00 EST 2018

DEAD box protein DDX1 promotes colorectal tumorigenesis through transcriptional activation of the LGR5 gene.

Cancer science

Tanaka K,Ikeda N,Miyashita K,Nuriya H,Hara T

Wed Aug 01 00:00:00 EDT 2018

DDX1 regulates alternative splicing and insulin secretion in pancreatic β cells.

Biochemical and biophysical research communications

Zhong W,Li Z,Zhou M,Xu T,Wang Y

Thu Jun 07 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

Thu May 17 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

Thu May 17 00:00:00 EDT 2018

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Journal of molecular biology

Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR

Fri Feb 16 00:00:00 EST 2018

BEX1 is an RNA-dependent mediator of cardiomyopathy.

Nature communications

Accornero F,Schips TG,Petrosino JM,Gu SQ,Kanisicak O,van Berlo JH,Molkentin JD

Thu Nov 30 00:00:00 EST 2017

RNase L activates the NLRP3 inflammasome during viral infections.

Cell host & microbe

Chakrabarti A,Banerjee S,Franchi L,Loo YM,Gale M,Núñez G,Silverman RH

Wed Apr 08 00:00:00 EDT 2015

DDX1 is an RNA-dependent ATPase involved in HIV-1 Rev function and virus replication.

Journal of molecular biology

Edgcomb SP,Carmel AB,Naji S,Ambrus-Aikelin G,Reyes JR,Saphire AC,Gerace L,Williamson JR

Fri Jan 06 00:00:00 EST 2012

Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1

Gene Aliases: AA409185; DBP-RB; DDX1; UKVH5d

UniProt ID: (Human) Q92499, (Rat) Q641Y8, (Mouse) Q91VR5

Entrez Gene ID: (Human) 1653, (Rat) 84474, (Mouse) 104721

Function(s)

spliceosomal complex assembly double-strand break repair transcription, DNA-templated regulation of transcription, DNA-templated tRNA splicing, via endonucleolytic cleavage and ligation regulation of translational initiation multicellular organism development response to virus RNA secondary structure unwinding DNA duplex unwinding response to exogenous dsRNA nucleic acid phosphodiester bond hydrolysis protein localization to cytoplasmic stress granule mRNA processing tRNA processing regulation of nucleic acid-templated transcription

Process(es)

DNA binding chromatin binding transcription cofactor activity RNA helicase activity double-stranded RNA binding ATP-dependent RNA helicase activity nuclease activity exonuclease activity protein binding ATP binding ATP-dependent helicase activity poly(A) binding DNA/RNA helicase activity poly(A) RNA binding nucleotide binding nucleic acid binding RNA binding helicase activity hydrolase activity

DDX1 Polyclonal Antibody

Product Details

11357-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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