Western blot analysis of HeLa Whole Cell Lysate using PA5-19537, DDX17 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19537 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human DDX17.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.2% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 14 kDa,70 kDa and 78 kDa.
This antibody is predicted to react with mouse, rat and dog based on sequence homology.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp translation initiation codon, have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.