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DDX3 Polyclonal Antibody

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Antibody Testing Data (22)

DDX3 Antibody in Immunocytochemistry (ICC/IF)

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DDX3 Antibody (11115-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (Product # 11115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DDX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDX3 Antibody in Immunoprecipitation (IP)

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Product Details

11115-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDX3 Fusion Protein Ag1614 (312-662 aa encoded by BC011819)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can bind both DDX3X and DDX3Y for the close sequences.

Immunogen sequence: DLERGCHLL VATPGRLVDM MERGKIGLDF CKYLVLDEAD RMLDMGFEPQ IRRIVEQDTM PPKGVRHTMM FSATFPKEIQ MLARDFLDEY IFLAVGRVGS TSENITQKVV WVEESDKRSF LLDLLNATGK DSLTLVFVET KKGADSLEDF LYHEGYACTS IHGDRSQRDR EEALHQFRSG KSPILVATAV AARGLDISNV KHVINFDLPS DIEEYVHRIG RTGRVGNLGL ATSFFNERNI NITKDLLDLL VEAKQEVPSW LENMAYEHHY KGSSRGRSKS SRFSGGFGAR DYRQSSGASS SSFSSSRASS SRSGGGGHGS SRGFGGGGYG GFYNSDGYGG NYNSQGVDWW GN (312-662 aa encoded by BC011819)

Target Information

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein.

Journal of virology

Bei C,Zhang C,Wu H,Feng H,Zhang YA,Tu J

Tue Feb 28 00:00:00 EST 2023

DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein.

Journal of virology

Bei C,Zhang C,Wu H,Feng H,Zhang YA,Tu J

Tue Feb 28 00:00:00 EST 2023

The involvement of DDX3X in compression-induced nucleus pulposus pyroptosis.

Biochemical and biophysical research communications

Chi S,Li S,Xu Z,Yang G,Song Y,Liao Z,Yang C,Wu X

Sun May 07 00:00:00 EDT 2023

The involvement of DDX3X in compression-induced nucleus pulposus pyroptosis.

Biochemical and biophysical research communications

Chi S,Li S,Xu Z,Yang G,Song Y,Liao Z,Yang C,Wu X

Sun May 07 00:00:00 EDT 2023

The involvement of DDX3X in compression-induced nucleus pulposus pyroptosis.

Biochemical and biophysical research communications

Chi S,Li S,Xu Z,Yang G,Song Y,Liao Z,Yang C,Wu X

Sun May 07 00:00:00 EDT 2023

The involvement of DDX3X in compression-induced nucleus pulposus pyroptosis.

Biochemical and biophysical research communications

Chi S,Li S,Xu Z,Yang G,Song Y,Liao Z,Yang C,Wu X

Sun May 07 00:00:00 EDT 2023

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cell

Cui Q,Bi H,Lv Z,Wu Q,Hua J,Gu B,Huo C,Tang M,Chen Y,Chen C,Chen S,Zhang X,Wu Z,Lao Z,Sheng N,Shen C,Zhang Y,Wu ZY,Jin Z,Yang P,Liu H,Li J,Bai G

Thu Feb 16 00:00:00 EST 2023

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cell

Cui Q,Bi H,Lv Z,Wu Q,Hua J,Gu B,Huo C,Tang M,Chen Y,Chen C,Chen S,Zhang X,Wu Z,Lao Z,Sheng N,Shen C,Zhang Y,Wu ZY,Jin Z,Yang P,Liu H,Li J,Bai G

Thu Feb 16 00:00:00 EST 2023

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Cell reports

Curdy N,Lanvin O,Cerapio JP,Pont F,Tosolini M,Sarot E,Valle C,Saint-Laurent N,Lhuillier E,Laurent C,Fournié JJ,Franchini DM

Tue Mar 28 00:00:00 EDT 2023

Long noncoding RNA RFPL1S-202 inhibits ovarian cancer progression by downregulating the IFN-β/STAT1 signaling.

Experimental cell research

Liu S,Chen X,Huang K,Xiong X,Shi Y,Wang X,Pan X,Cong Y,Sun Y,Ge L,Xu J,Jia X

Sun Jan 15 00:00:00 EST 2023

Circular EZH2-encoded EZH2-92aa mediates immune evasion in glioblastoma via inhibition of surface NKG2D ligands.

Nature communications

Zhong J,Yang X,Chen J,He K,Gao X,Wu X,Zhang M,Zhou H,Xiao F,An L,Wang X,Shi Y,Zhang N

Mon Aug 15 00:00:00 EDT 2022

Overexpressed DDX3x promotes abdominal aortic aneurysm formation and activates AKT in ApoE knockout mice.

Biochemical and biophysical research communications

Zhou Y,Chai H,Hu Y,Liu R,Jiang H,Fan R,Chen W,Chen X,Huang F

Sat Dec 17 00:00:00 EST 2022

Overexpressed DDX3x promotes abdominal aortic aneurysm formation and activates AKT in ApoE knockout mice.

Biochemical and biophysical research communications

Zhou Y,Chai H,Hu Y,Liu R,Jiang H,Fan R,Chen W,Chen X,Huang F

Sat Dec 17 00:00:00 EST 2022

DDX3 acts as a tumor suppressor in colorectal cancer as loss of DDX3 in advanced cancer promotes tumor progression by activating the MAPK pathway.

International journal of biological sciences

Shen L,Zhang J,Xu M,Zheng Y,Wang M,Yang S,Qin B,Li S,Dong L,Dai F

Tue Jul 19 00:00:00 EDT 2022

DDX3 acts as a tumor suppressor in colorectal cancer as loss of DDX3 in advanced cancer promotes tumor progression by activating the MAPK pathway.

International journal of biological sciences

Shen L,Zhang J,Xu M,Zheng Y,Wang M,Yang S,Qin B,Li S,Dong L,Dai F

Tue Jul 19 00:00:00 EDT 2022

DDX3X alleviates doxorubicin-induced cardiotoxicity by regulating Wnt/β-catenin signaling pathway in an in vitro model.

Journal of biochemical and molecular toxicology

Feng D,Li J,Guo L,Liu J,Wang S,Ma X,Song Y,Liu J,Hao E

Mon Aug 01 00:00:00 EDT 2022

DEAD-Box Helicase 3 X-Linked Promotes Metastasis by Inducing Epithelial-Mesenchymal Transition via p62/Sequestosome-1.

Digestive diseases and sciences

Zheng Y,Xu B,Zhao Y,Yang S,Wang S,Ma L,Dong L

Mon Nov 01 00:00:00 EDT 2021

DEAD-Box Helicase 3 X-Linked Promotes Metastasis by Inducing Epithelial-Mesenchymal Transition via p62/Sequestosome-1.

Digestive diseases and sciences

Zheng Y,Xu B,Zhao Y,Yang S,Wang S,Ma L,Dong L

Mon Nov 01 00:00:00 EDT 2021

Aluminum impairs cognitive function by activating DDX3X-NLRP3-mediated pyroptosis signaling pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Hao W,Hao C,Wu C,Xu Y,Wu S,Lu X,Yang J,Jin C

Mon Nov 01 00:00:00 EDT 2021

Aluminum impairs cognitive function by activating DDX3X-NLRP3-mediated pyroptosis signaling pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Hao W,Hao C,Wu C,Xu Y,Wu S,Lu X,Yang J,Jin C

Mon Nov 01 00:00:00 EDT 2021

DDX3X deficiency alleviates LPS-induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome.

Experimental and therapeutic medicine

Feng D,Guo L,Liu J,Song Y,Ma X,Hu H,Liu J,Hao E

Wed Dec 01 00:00:00 EST 2021

Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development.

Neuron

Lennox AL,Hoye ML,Jiang R,Johnson-Kerner BL,Suit LA,Venkataramanan S,Sheehan CJ,Alsina FC,Fregeau B,Aldinger KA,Moey C,Lobach I,Afenjar A,Babovic-Vuksanovic D,Bézieau S,Blackburn PR,Bunt J,Burglen L,Campeau PM,Charles P,Chung BHY,Cogné B,Curry C,D'Agostino MD,Di Donato N,Faivre L,Héron D,Innes AM,Isidor B,Keren B,Kimball A,Klee EW,Kuentz P,Küry S,Martin-Coignard D,Mirzaa G,Mignot C,Miyake N,Matsumoto N,Fujita A,Nava C,Nizon M,Rodriguez D,Blok LS,Thauvin-Robinet C,Thevenon J,Vincent M,Ziegler A,Dobyns W,Richards LJ,Barkovich AJ,Floor SN,Silver DL,Sherr EH

Wed May 06 00:00:00 EDT 2020

CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation.

Neuron

Cheng W,Wang S,Zhang Z,Morgens DW,Hayes LR,Lee S,Portz B,Xie Y,Nguyen BV,Haney MS,Yan S,Dong D,Coyne AN,Yang J,Xian F,Cleveland DW,Qiu Z,Rothstein JD,Shorter J,Gao FB,Bassik MC,Sun S

Wed Dec 04 00:00:00 EST 2019

Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.

Genes & genomics

Ahmad A,Zhang W,Wu M,Tan S,Zhu T

Thu Mar 01 00:00:00 EST 2018

Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.

Genes & genomics

Ahmad A,Zhang W,Wu M,Tan S,Zhu T

Thu Mar 01 00:00:00 EST 2018

(DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.

Oncotarget

Wang X,Wang R,Luo M,Li C,Wang HX,Huan CC,Qu YR,Liao Y,Mao X

Tue May 16 00:00:00 EDT 2017

(DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.

Oncotarget

Wang X,Wang R,Luo M,Li C,Wang HX,Huan CC,Qu YR,Liao Y,Mao X

Tue May 16 00:00:00 EDT 2017

(DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.

Oncotarget

Wang X,Wang R,Luo M,Li C,Wang HX,Huan CC,Qu YR,Liao Y,Mao X

Tue May 16 00:00:00 EDT 2017

(DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.

Oncotarget

Wang X,Wang R,Luo M,Li C,Wang HX,Huan CC,Qu YR,Liao Y,Mao X

Tue May 16 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX3X; CAP-Rf; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 3; D1Pas1-related sequence 2; DBX; DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 3; DEAD box protein 3, X-chromosomal; DEAD box RNA helicase DEAD3; DEAD box, X isoform; DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked; DEAD/H box-3; Embryonic RNA helicase; fibroblast growth factor inducible 14; Helicase-like protein 2; HLP2; mDEAD3

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Gene Aliases: CAP-Rf; D1Pas1-rs2; DBX; DDX14; DDX3; DDX3X; Dead3; Erh; Fin14; HLP2; MRX102

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UniProt ID: (Human) O00571, (Mouse) Q62167

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Entrez Gene ID: (Human) 1654, (Rat) 317335, (Mouse) 13205

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Function(s)

DNA binding RNA binding GTPase activity ATP-dependent DNA helicase activity ATP-dependent RNA helicase activity protein binding ATP binding transcription factor binding poly(A) binding eukaryotic initiation factor 4E binding ATPase activity nucleoside-triphosphatase activity translation initiation factor binding RNA stem-loop binding ribosomal small subunit binding CTPase activity poly(A) RNA binding mRNA 5'-UTR binding cadherin binding involved in cell-cell adhesion nucleotide binding nucleic acid binding helicase activity hydrolase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDX3 Polyclonal Antibody

DDX3 Antibody (11115-1-AP) in ICC/IF

Product Details

11115-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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