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DDX39B Polyclonal Antibody

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DDX39B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

DDX39B Antibody in Western Blot (WB) — Rat spleen tissue were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDX39B Antibody in Immunoprecipitation (IP) — IP result of anti-UAP56 (IP:14798-1-AP, 4ug; Detection:14798-1-AP 1:500) with Jurkat cells lysate 1200ug.

Product Details

14798-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DDX39B Fusion Protein Ag6512 (80-428 aa encoded by BC000361) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody may cross-react with DDX39A.

Immunogen sequence: ILGMDVLCQ AKSGMGKTAV FVLATLQQLE PVTGQVSVLV MCHTRELAFQ ISKEYERFSK YMPNVKVAVF FGGLSIKKDE EVLKKNCPHI VVGTPGRILA LARNKSLNLK HIKHFILDEC DKMLEQLDMR RDVQEIFRMT PHEKQVMMFS ATLSKEIRPV CRKFMQDPME IFVDDETKLT LHGLQQYYVK LKDNEKNRKL FDLLDVLEFN QVVIFVKSVQ RCIALAQLLV EQNFPAIAIH RGMPQEERLS RYQQFKDFQR RILVATNLFG RGMDIERVNI AFNYDMPEDS DTYLHRVARA GRFGTKGLAI TFVSDENDAK ILNDVQDRFE VNISELPDEI DISSYIEQTR (80-428 aa encoded by BC000361)

Target Information

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.

Scientific reports

Boehringer A,Garcia-Mansfield K,Singh G,Bakkar N,Pirrotte P,Bowser R

Mon Nov 06 00:00:00 EST 2017

ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.

Scientific reports

Boehringer A,Garcia-Mansfield K,Singh G,Bakkar N,Pirrotte P,Bowser R

Mon Nov 06 00:00:00 EST 2017

ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.

Scientific reports

Boehringer A,Garcia-Mansfield K,Singh G,Bakkar N,Pirrotte P,Bowser R

Mon Nov 06 00:00:00 EST 2017

The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent nucleocytoplasmic shuttling activity.

PloS one

Thomas M,Lischka P,Müller R,Stamminger T

Tue Dec 06 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; D17H6S81E-1; D20H6S81e; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B; DEAD box protein UAP56; HLA-B associated transcript 1; HLA-B associated transcript 1A; HLA-B-associated transcript 1 protein; HLA-B-associated transcript 1A; Nuclear RNA helicase; nuclear RNA helicase (DEAD family); nuclear RNA helicase Bat1; spliceosome RNA helicase BAT1; Spliceosome RNA helicase DDX39B

Gene Aliases: 0610030D10Rik; AI428441; Bat-1; BAT1; Bat1a; D17H6S81E; D17H6S81E-1; D6S81E; D6S81Eh; DDX39B; p47; UAP56

UniProt ID: (Human) Q5STU4, (Rat) Q63413, (Mouse) Q9Z1N5

Entrez Gene ID: (Human) 7919, (Rat) 114612, (Mouse) 53817

Function(s)

spliceosomal complex assembly mRNA splicing, via spliceosome liver development termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus RNA splicing RNA secondary structure unwinding mRNA 3'-end processing positive regulation of DNA-templated transcription, elongation positive regulation of translation viral mRNA export from host cell nucleus positive regulation of cell growth involved in cardiac muscle cell development positive regulation of vascular smooth muscle cell proliferation negative regulation of DNA damage checkpoint positive regulation of DNA biosynthetic process mRNA processing transport cellular response to DNA damage stimulus regulation of gene expression mRNA transport

Process(es)

ATP-dependent RNA helicase activity protein binding ATP binding RNA-dependent ATPase activity ATPase activity U6 snRNA binding U4 snRNA binding protein complex binding ATP-dependent protein binding poly(A) RNA binding nucleotide binding nucleic acid binding RNA binding helicase activity hydrolase activity

DDX39B Polyclonal Antibody

Product Details

14798-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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