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Invitrogen
DDX4 Polyclonal Antibody
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FIGURE: 1 / 13
DDX4 Antibody (PA5-79147) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of DDX4 in U20S cells using DDX4 Polyclonal Antibody (Product # PA5-79147). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL. DyLight 594 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets ... View More
Product Details
PA5-79147
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DDX4 (253-272aa EDEDSIFAHYQTGINFDKYD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat testis tissue, mouse testis tissue. IHC: Rat Ovary tissue, Rat Testis tissue, human ovarian cancer tissue IHC-F: Rat Intestine Tissue. ICC/IF: U20S cell, MCF-7 Cell. Flow: PC-3 cell, SiHa cell.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX4; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 4; DEAD box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; Mvh; mvh / m'vasa; Probable ATP-dependent RNA helicase DDX4; putative ATP-dependent RNA helicase DDX4; rVLG; Vasa homolog; vasa-like gene protein
Gene Aliases: AV206478; DDX4; Mvh; RVLG; VASA
UniProt ID: (Human) Q9NQI0, (Rat) Q64060, (Mouse) Q61496
Entrez Gene ID: (Human) 54514, (Rat) 310090, (Mouse) 13206
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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