Invitrogen

DDX5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (29) DDX5 antibodies

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Datasheet

Protocols

Questions & Answers

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DDX5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of DDX5 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DDX5 Polyclonal Antibody (Product # PA1-31019) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to the nucleus. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

DDX5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of DDX5 in human prostate tissue using DDX5 Polyclonal Antibody (Product # PA1-31019) at a dilution of 2.5 µg/mL. Antigen retrieval : citrate buffer pH 6.

DDX5 Antibody in Western Blot (WB) — Western blot was performed using Anti-DDX5 Polyclonal Antibody (Product # PA1-31019) and a 60 kDa band corresponding to DDX5 was observed across cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2),Hep G2 (Lane 3), MCF7 (Lane 4), HT-29 (Lane 5), NIH/3T3 (Lane 6) and Rat testis (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit Anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

DDX5 Antibody in RNA Immunoprecipitation (RIP) — RNA Immunoprecipitation (RIP) assay of endogenous DDX5 protein using Anti-DDX5 Antibody: RIP assay was performed using Anti-DDX5 Polyclonal Antibody (Product # PA1-31019, 5 ug) on whole cell lysate from HeLa cells. Normal IgG was used as a negative IP control. RNA purified by RiboPure™ RNA Purification Kit (Product # AM1924) was analyzed by RT-PCR using the Power SYBR® Green RNA-to-CT™ 1-Step Kit (Product # 4389986) with the primers pairs over U1 snRNA, ACTB, MALAT non-coding RNA and 5S rRNA. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

DDX5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with DDX5 siRNA and decrease in signal intensity was observed in western blot application using DDX5 Polyclonal Antibody (Product # PA1-31019). {KD}

Product Details

PA1-31019

Applications	Tested Dilution	Publications
Western Blot (WB)	0.3-1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	1:100	-
RNA Immunoprecipitation (RIP)	2.5 µg/10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	synthetic peptide PMIGYPMPTGYSQ. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2230422

Product Specific Information

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX10261-PEP)..

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX5; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD box protein 5; DEAD box RNA helicase DEAD1; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DKFZp434E109; DKFZp686J01190; mDEAD1; MGC118083; p68 RNA helicase; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68; RP23-247J12.4

Gene Aliases: 2600009A06Rik; DDX5; G17P1; HELR; HLR1; HUMP68; p68

UniProt ID: (Human) B4DLW8

Entrez Gene ID: (Human) 1655, (Mouse) 13207

Function(s)

negative regulation of transcription from RNA polymerase II promoter regulation of alternative mRNA splicing, via spliceosome mRNA splicing, via spliceosome mRNA transcription RNA secondary structure unwinding cell growth positive regulation of intracellular estrogen receptor signaling pathway positive regulation of DNA damage response, signal transduction by p53 class mediator regulation of viral genome replication regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter rhythmic process regulation of androgen receptor signaling pathway intrinsic apoptotic signaling pathway by p53 class mediator regulation of skeletal muscle cell differentiation in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing circadian rhythm RNA splicing positive regulation of transcription, DNA-templated

Process(es)

transcription coactivator activity RNA helicase activity ATP-dependent RNA helicase activity protein binding calmodulin binding ATP binding enzyme binding estrogen receptor binding pre-mRNA binding poly(A) RNA binding calcium-dependent protein binding androgen receptor binding nucleotide binding nucleic acid binding transcription cofactor activity RNA binding helicase activity hydrolase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

DDX5 Polyclonal Antibody

Product Details

PA1-31019

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

RNA Immunoprecipitation (RIP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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