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Invitrogen
DDX5 Polyclonal Antibody
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FIGURE: 1 / 4
DDX5 Antibody (PA5-89140) in IHC (P)
Immunohistochemistry analysis of DDX5 in paraffin-embedded human lung cancer. Samples were incubated with DDX5 Polyclonal antibody (Product # PA5-89140) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-89140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_0043871)
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSGYSSDRDR GRDRGFGAPR FGGSRAGPLS GKKFGNPGEK LVKKKWNLDE LPKFEKNFYQ EHPDLARRTA QEVETYRRSK EITVRGHNCP KPVLNFYEAN FPANVMDVIA RQNFTEPTAI QAQGWPVALS GLDMVGVAQT GSGKTLSYLL PAIVHINHQP FLERGDGPIC LVLAPTRELA QQVQQVAAEY CRACRLKSTC IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR RTTYLVLDEA DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET KRRCDELTRK MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV ASRGLDVEDV KFVINYDYPN SSEDYIHRIG RTARSTKTGT AYTFFTPNNI KQVSDLISVL REANQAINPK LLQLVEDRGS GRSRGRGGMK DDRRDRYSAG KRGGFNTFRD RENYDRGYSS LLKRDFGAKT QNGVYSAANY TNGSFGSNFV SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VPNMHNGMNQ QAYAYPATAA APMIGYPMPT GYSQ; Positive Samples: HepG2, 293T, SH-SY5Y, Jurkat, Mouse brain, Rat testis; Cellular Location: Nucleus, nucleolus
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX5; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 5; ddx5 gene protein; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD box protein 5; DEAD box RNA helicase DEAD1; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DKFZp434E109; DKFZp686J01190; mDEAD1; MGC118083; p68 RNA helicase; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68; RP23-247J12.4
Gene Aliases: 2600009A06Rik; DDX5; G17P1; HELR; HLR1; HUMP68; p68
UniProt ID: (Human) P17844
Entrez Gene ID: (Human) 1655, (Mouse) 13207, (Rat) 287765
transcription coactivator activity
RNA helicase activity
ATP-dependent RNA helicase activity
protein binding
calmodulin binding
ATP binding
enzyme binding
estrogen receptor binding
pre-mRNA binding
poly(A) RNA binding
calcium-dependent protein binding
androgen receptor binding
nucleotide binding
nucleic acid binding
transcription cofactor activity
RNA binding
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
negative regulation of transcription from RNA polymerase II promoter
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
mRNA transcription
RNA secondary structure unwinding
cell growth
positive regulation of intracellular estrogen receptor signaling pathway
positive regulation of DNA damage response, signal transduction by p53 class mediator
regulation of viral genome replication
regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of androgen receptor signaling pathway
intrinsic apoptotic signaling pathway by p53 class mediator
regulation of skeletal muscle cell differentiation
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA processing
circadian rhythm
RNA splicing
positive regulation of transcription, DNA-templated
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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