|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 553-598 of human DDX52|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 40 kDa.
Purity is >95% by SDS-PAGE.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box protein 52 (DDX52), also known as ATP-dependent RNA helicase ROK1-like or HUSSY-19, is a 599 amino acid protein belonging to the DEAD box helicase family. Localized to the nucleus, DDX52 is phosphorylated by ATM or ATR upon DNA damage. DDX52 contains one helicase ATP-binding domain and one helicase C-terminal domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP dependent RNA helicase ROK1 like; ATP-dependent RNA helicase ROK1-like; DDX52; DEAD (Asp-Glu-Ala-Asp) box polypeptide 52; DEAD box protein 52; Probable ATP dependent RNA helicase DDX52; Probable ATP-dependent RNA helicase DDX52; ROK1
Gene Aliases: DDX52; HUSSY-19; HUSSY19; ROK1
UniProt ID: (Human) Q9Y2R4
Entrez Gene ID: (Human) 11056
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.