Immunofluorescent analysis of DDX6 showing staining in the cytoplasm and P body of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a DDX6 polyclonal antibody (Product # PA5-27786) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 236 of Human DDX6|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27786 targets DDX6 in IHC (P), IF/ICC, and WB applications and shows reactivity with Human samples.
The PA5-27786 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 236 of Human DDX6.
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.
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