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Immunofluorescent analysis of DECR2 showing staining in the peroxisome of A549 cells. A549 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a DECR2 polyclonal antibody (Product # PA5-30503) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 92 and 242 of Human DECR2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30503 targets DECR2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30503 immunogen is recombinant protein fragment corresponding to a region within amino acids 92 and 242 of Human DECR2.
Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
2; 2,4-dienoyl CoA reductase 2, peroxisomal; 2,4-dienoyl-CoA reductase 2; 4-dienoyl-CoA reductase; 4-dienoyl-CoA reductase 2; member 1; PDCR; peroxisomal 2; SDR17C1; short chain dehydrogenase/reductase family 17C; short chain dehydrogenase/reductase family 17C member 1; short chain dehydrogenase/reductase family 17C, member 1
DECR2; PDCR; SDR17C1