Immunofluorescent analysis of DEPDC1 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a DEPDC1 polyclonal antibody (Product # PA5-34864) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 612 and 811 of DEPDC1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34864 targets DEPDC1 in WB applications and shows reactivity with Human samples.
The PA5-34864 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 612 and 811 of DEPDC1.
May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.