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Invitrogen
DFFB Polyclonal Antibody
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Product Details
PA5-19913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to amino acids near the center of murine CAD
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot applications, this antibody detects a band at ~40kDa. A suggested positive control is K562 cell lysate.
PA5-19913 can be used with blocking peptide PEP-0039.
Antibodies should not be exposed to prolonged high temperatures.
Target Information
Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, or UV irradiation, anticancer drugs, and survival factor deprivation. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2410008J01Rik; AU018859; c-truncated CAD; CAD; Caspase-activated deoxyribonuclease; caspase-activated DNase; Caspase-activated nuclease; CIDE-N-only protein; Cpad; CPAN; DFF-2; DFF-40; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase); DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, 40 kD, beta subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta subunit; DNase inhibited by DNA fragmentation factor; RP13-531C17.1; RP23-254N4.2; unnamed protein product
Gene Aliases: 40kDa; 5730477D02Rik; CAD; CPAN; DFF-40; DFF2; DFF40; DFFB; Didff
UniProt ID: (Human) O76075, (Rat) Q99N34, (Mouse) O54788
Entrez Gene ID: (Human) 1677, (Rat) 84359, (Mouse) 13368
DNA binding
nuclease activity
endodeoxyribonuclease activity
deoxyribonuclease activity
protein binding
hydrolase activity
enzyme binding
protein domain specific binding
identical protein binding
disordered domain specific binding
GPI-anchor transamidase activity
coenzyme F390-A hydrolase activity
coenzyme F390-G hydrolase activity
DNA metabolism protein
nucleic acid metabolism protein
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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