Invitrogen

DFFB Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (10) DFFB antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

DFFB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of DFF40 in K562 cells with DFFB Polyclonal Antibody (Product # PA5-19913) at 20 µg/mL. Green: DFF40 Blue: DAPI staining

DFFB Antibody in Western Blot (WB) — Western Blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFFB Polyclonal Antibody (Product # PA5-19913) (I18) at 1:500 dilution.

DFFB Antibody — Figure 3 Mitotic telomere damage is dependent on caspase-9, caspase-7 and the endonuclease CAD. ( A , B ) U2OS cells including those expressing a dominant inhibitory caspase-9 mutant (C287A) were treated with nocodazole and at least 50 cells were analysed in each of three independent experiments. Representative microscopic fields for ( B) are shown in ( A ). ( C , D) U2OS cells were transfected with siRNA oligos targeting caspase-3 (C-3), caspase-7 (C-7) ( C) , the endonuclease CAD ( D ) or a control siRNA (luciferase, Ctrl). The mean number of gammaH2AX foci per cell is represented in the graph (data from three independent experiments in which at least 50 cells were analysed). Lower panels, Western blots with the indicated antibodies showing depletion of the targeted proteins. {KD}

Product Details

PA5-19913

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A peptide corresponding to amino acids near the center of murine CAD
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_11153625

Product Specific Information

In Western blot applications, this antibody detects a band at ~40kDa. A suggested positive control is K562 cell lysate.

PA5-19913 can be used with blocking peptide PEP-0039.

Target Information

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, or UV irradiation, anticancer drugs, and survival factor deprivation. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prolonged mitotic arrest induces a caspase-dependent DNA damage response at telomeres that determines cell survival.

Scientific reports

Hain KO,Colin DJ,Rastogi S,Allan LA,Clarke PR

PA5-19913 was used in western blot to study induction of a caspase-dependent DNA damage response at telomeres that determine cell survival by prolonged mitotic arrest

Fri May 27 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 2410008J01Rik; AU018859; CAD; Caspase-activated deoxyribonuclease; caspase-activated DNase; Caspase-activated nuclease; Cpad; CPAN; DFF-2; DFF-40; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase); DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, 40 kD, beta subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta subunit; DNase inhibited by DNA fragmentation factor; RP13-531C17.1; RP23-254N4.2

Gene Aliases: 40kDa; 5730477D02Rik; CAD; CPAN; DFF-40; DFF2; DFF40; DFFB; Didff

UniProt ID: (Human) O76075, (Rat) Q99N34, (Mouse) O54788

Entrez Gene ID: (Human) 1677, (Rat) 84359, (Mouse) 13368

Molecular Function: DNA metabolism protein nucleic acid metabolism protein