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Invitrogen
DGAT2 Polyclonal Antibody
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FIGURE: 1 / 3
DGAT2 Antibody (PA5-103785) in ICC/IF
Immunofluorescent analysis of DGAT2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DGAT2 polyclonal antibody (Product # PA5-103785) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-103785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DGAT2(Accession Q96PD7), corresponding to amino acid residues N155-R205.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total DGAT2.
Target Information
Acyl-coenzyme A:diacylglycerol acyltransferase, also known as DGAT1 and ARGP1, is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT1 catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT1 plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism, such as intestinal fat absorption, lipoprotein assembly, adipose tissue form-ation and lactation. DGAT2, which has no homology to DGAT1, differs from DGAT1 in that its activity has been shown to be inhibited by MgCl in an in vitro assay. DGAT2 is expressed primarily in liver and white adipose tissue, which suggests that it plays an important role in mammalian triglyceride metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acyl-CoA retinol O-fatty-acyltransferase; ARAT; diacylglycerol acyltransferase 2; Diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; Diglyceride acyltransferase 2; DKFZp686A15125; retinol O-fatty-acyltransferase
Gene Aliases: 0610010B06Rik; ARAT; DGAT-2; DGAT2; GS1999FULL; HMFN1045; UNQ738/PRO1433
UniProt ID: (Human) Q96PD7, (Rat) Q5FVP8, (Mouse) Q9DCV3
Entrez Gene ID: (Human) 84649, (Rat) 252900, (Mouse) 67800
2-acylglycerol O-acyltransferase activity
diacylglycerol O-acyltransferase activity
protein homodimerization activity
retinol O-fatty-acyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
acyltransferase
glycerol metabolic process
positive regulation of triglyceride biosynthetic process
triglyceride biosynthetic process
lipid storage
low-density lipoprotein particle clearance
long-chain fatty-acyl-CoA metabolic process
cellular triglyceride homeostasis
acylglycerol acyl-chain remodeling
retinol metabolic process
cholesterol homeostasis
positive regulation of gluconeogenesis
negative regulation of fatty acid oxidation
diacylglycerol metabolic process
regulation of lipoprotein metabolic process
fatty acid homeostasis
fat pad development
cellular response to oleic acid
regulation of cholesterol metabolic process
regulation of plasma lipoprotein particle levels
lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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