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Invitrogen
DGKD Polyclonal Antibody
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FIGURE: 1 / 2
DGKD Antibody (PA5-75256) in IHC (P)
Immunohistochemistry analysis of DGKD in paraffin-embedded human rectum carcinoma tissue (cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with DGKD polyclonal antibody (Product # PA5-75256) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Product Details
PA5-75256
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 34-88 of Human DGKD.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-alpha influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-beta is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-gamma encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-delta is most abundant in skeletal muscle. DGK-epsilon shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis. DGK-theta is most abundant in the cerebellum and hippocampus. DGK-iota is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb. DGK-eta is closely related to DGK-delta. DGK-zeta is most abundant in brain and muscle. DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 130 kDa diacylglycerol kinase; DAG kinase delta; DGK delta; DGK-delta; Diacylglycerol kinase delta; diacylglycerol kinase, delta; diacylglycerol kinase, delta 130kDa; Diglyceride kinase; Diglyceride kinase delta; EC 2.7.1.107; FLJ26930; unnamed protein product
Gene Aliases: AI841987; D330025K09; DGK-delta; DGKD; dgkd-2; DGKdelta; KIAA0145; RGD1563309
UniProt ID: (Human) Q16760, (Mouse) E9PUQ8
Entrez Gene ID: (Human) 8527, (Mouse) 227333, (Rat) 368088
nucleotide binding
diacylglycerol kinase activity
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
kinase binding
diacylglycerol binding
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
NAD+ kinase activity
kinase
lipid metabolic process
phosphatidic acid biosynthetic process
endocytosis
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
protein transport
platelet activation
intracellular signal transduction
positive regulation of epidermal growth factor receptor signaling pathway
diacylglycerol metabolic process
diacylglycerol catabolic process
phosphatidic acid metabolic process
glycerolipid metabolic process
lipid phosphorylation
negative regulation of phospholipase C/protein kinase C signal transduction
positive regulation of clathrin-mediated endocytosis
response to organic substance
phosphorylation
protein homooligomerization
protein kinase C-activating G-protein coupled receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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