Immunofluorescent analysis using a DGKZ polyclonal antibody (Product # PA5-15319). Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with a SF1 antibody and a DGKZ polyclonal antibody (Product # PA5-15319) (B) for 1 h. Coverslips were washed and incubated with a fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellularsignaling cascade and signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.