1 cm 2 of a leaf from Arabidopsis thaliana Col-0 (1) and or t-DNA insertion lines dhar 1-1 (2), dhar 1-2 (3), dhar 1-3 (4), dhar 2-1 (5), dhar 2-2 (6), dhar 1-3 EOS-DHAR 1 (7), was extracted using 200 µL Lyse& Load-Buffer (Grefen et al. 2009). 10 µL were separated on a 15% SDS-PAGE and blotted 1 h to PVDF (using Bjerrum Buffer in a semidry blot). Blots were blocked with 5% Milk in 1 x TBS-Tween 20 (1%) for ... View More

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Product Details

AS11-1747

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

Product Specifications

Species Reactivity

Plant

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

200 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

For reconstitution add 200 µL of sterile water.

Specific Species Reactivity: Arabidopsis thaliana

Target Information

DHAR2 encodes a soluble, predominantly cytosolic dehydroascorbate reductase that functions in the ascorbate-glutathione cycle to maintain cellular redox homeostasis by catalyzing the glutathione-dependent reduction of dehydroascorbate back to ascorbate, thereby supporting antioxidant capacity during oxidative stress. The protein belongs to the glutathione transferase superfamily and adopts the canonical GST-like architecture, comprising an N-terminal thioredoxin-like domain that contributes to glutathione binding and a largely all-helical C-terminal domain; structural and mechanistic analyses indicate it acts as a monomer and relies on a conserved catalytic cysteine that undergoes reversible redox chemistry during turnover, consistent with a ping-pong-type mechanism involving sequential interaction with glutathione and dehydroascorbate. Genetic and cell biology studies support a role for DHAR2 in shaping glutathione/ascorbate redox states in vivo, with loss-of-function combinations affecting oxidative stress-linked signaling outputs, including salicylic acid pathway activation under elevated oxidative load.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AT1G75270; dehydroascorbate reductase 2; F22H5.1; F22H5_1

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DHAR2/Dehydroascorbate Reductase 2 Polyclonal Antibody

DHAR2/Dehydroascorbate Reductase 2 Antibody (AS11-1747) in WB