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DHFR Antibody (H00001719-D01P) in WB
Western Blot analysis of DHFR expression in transfected 293T cell line (H00001719-T01) by DHFR MaxPab polyclonal antibody.
Lane 1: DHFR transfected lysate(21.50 KDa).
Lane 2: Non-transfected lysate.
Lane 1: DHFR transfected lysate(21.50 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00001719-D01P
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
DHFR (XP_937759.1, 1 a.a. approximately 187 a.a) full-length human protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
Sequence of this protein is as follows: MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
Target Information
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dihydrofolate reductase
Gene Aliases: DHFR; DHFRP1; DYR
UniProt ID: (Human) P00374
Entrez Gene ID: (Human) 1719
Molecular Function:
reductase
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
glycine biosynthetic process
tetrahydrobiopterin biosynthetic process
one-carbon metabolic process
nucleotide biosynthetic process
axon regeneration
response to methotrexate
dihydrofolate metabolic process
tetrahydrofolate metabolic process
tetrahydrofolate biosynthetic process
folic acid metabolic process
positive regulation of nitric-oxide synthase activity
oxidation-reduction process
regulation of removal of superoxide radicals
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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