Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-0.5 µg/mL | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
0.5-1 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
E.coli-derived human DHFR recombinant protein (Position: V2-D187). |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
500 µg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS with 5mg BSA |
Contains |
0.05mg sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2746268 |
Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dihydrofolate reductase; Dihydrofolate reductase 1 (active)
Gene Aliases: 8430436I03Rik; AA607882; AI662710; AW555094; DHFR; Dhfr1; DHFRP1; DYR
UniProt ID: (Human) P00374, (Mouse) P00375, (Rat) Q920D2
Entrez Gene ID: (Human) 1719, (Mouse) 13361, (Rat) 24312
Molecular Function:
reductase
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