Invitrogen

DHFR Polyclonal Antibody

View all (16) DHFR antibodies

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Questions & Answers

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DHFR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DHFR on paraffin-embedded human lung cancer tissue. Sample was incubated with DHFR polyclonal antibody (Product# PA5-79152).

DHFR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DHFR on paraffin-embedded mouse brain tissue. Sample was incubated with DHFR polyclonal antibody (Product# PA5-79152).

DHFR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DHFR on paraffin-embedded rat brain tissue. Sample was incubated with DHFR polyclonal antibody (Product# PA5-79152).

DHFR Antibody in Western Blot (WB) — Western blot analysis of DHFR in Lane 1: rat liver tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: HepG2 whole cell lysate, Lane 4: Jurkat whole cell lysate using 40-50 µg per well. Sample was incubated with DHFR (Product # PA5-79152) at a dilution of 0.5 µg/mL.

DHFR Antibody in Western Blot (WB) — Western blot analysis of DHFR in recombinant human DHFR protein using 0.5 ng per well. Sample was incubated with DHFR polyclonal antibody (Product # PA5-79152) at a dilution of 0.5 µg/mL.

Product Details

PA5-79152

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human DHFR recombinant protein (Position: V2-D187). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5mg BSA
Contains	0.05mg sodium azide
Storage conditions	-20°C
RRID	AB_2746268

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Dihydrofolate reductase; Dihydrofolate reductase 1 (active)

Gene Aliases: 8430436I03Rik; AA607882; AI662710; AW555094; DHFR; Dhfr1; DHFRP1; DYR

UniProt ID: (Human) P00374, (Mouse) P00375, (Rat) Q920D2

Entrez Gene ID: (Human) 1719, (Mouse) 13361, (Rat) 24312

Molecular Function: reductase

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle glycine biosynthetic process tetrahydrobiopterin biosynthetic process one-carbon metabolic process nucleotide biosynthetic process axon regeneration response to methotrexate dihydrofolate metabolic process tetrahydrofolate metabolic process tetrahydrofolate biosynthetic process folic acid metabolic process positive regulation of nitric-oxide synthase activity oxidation-reduction process regulation of removal of superoxide radicals response to nicotine

Process(es)

mRNA binding dihydrofolate reductase activity folic acid binding drug binding folate reductase activity methotrexate binding NADPH binding RNA binding oxidoreductase activity NADP binding dihydrofolic acid binding